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2020 Volume 5
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ARTICLE   Open Access    

Evaluation of agro-based waste substrates for micropropagule formation in biocontrol fungi, Trichoderma asperellum and T. harzianum

  • 1.

    Department of Biology, Faculty of Science, Universiti Putra Malaysia, 43400 Serdang Selangor, Malaysia

  • 2.

    Department of Botany, Faculty of Science, Omar Al Mukhtar University, Al Bayda, Libya

  • 3.

    Department of Microbiology, Faculty of Biotechnology and Biomolecular Sciences, Universiti Putra Malaysia, 43400 Serdang, Selangor, Malaysia

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  • Trichoderma species have shown efficiency on biocontrol of phytopathogens. For commercial application, it must be propagated in mass scale using a cost-effective method. As an alternative way to effectively deliver biocontrol fungi inoculum to the field; seven agro-based wastes including rice bran, biochar, empty fruit bunches, coconut fibre, compost, topsoil and mixed soil were used in this study for evaluating mass multiplication of Trichoderma species. Based on the evaluation of colony-forming units (cfu) among the agro-based waste media used, coconut fibre is the most suitable in promoting the sporulation of Trichoderma asperellum and T. harzianum. Trichoderma asperellum C1667 showed the higher micropropagule count through incubation period compared to T. harzianum C1675. After 120 days on the agro-based waste media, T. asperellum C1667 and T. harzianum C1675 produced the highest (7.717×105 cfu/g and 6.836±13.79×105 cfu/g) coconut fibres, respectively. Meanwhile, the mixed soil appeared with the lowest cfu. Coconut fibres were shown as a great biocomposting medium for both Trichoderma species. Findings of the present study are valuable for disease management using agro-based wastes as a cost-effective medium for biocontrol agents like Trichoderma species.
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    ZAE Hasan, NAI Mohd Zainudin, A Aris, MH Ibrahim, MT Yusof. 2020. Evaluation of agro-based waste substrates for micropropagule formation in biocontrol fungi, Trichoderma asperellum and T. harzianum. Studies in Fungi 5(1):94-102 doi: 10.5943/sif/5/1/9
    ZAE Hasan, NAI Mohd Zainudin, A Aris, MH Ibrahim, MT Yusof. 2020. Evaluation of agro-based waste substrates for micropropagule formation in biocontrol fungi, Trichoderma asperellum and T. harzianum. Studies in Fungi 5(1):94-102 doi: 10.5943/sif/5/1/9
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ARTICLE   Open Access    

Evaluation of agro-based waste substrates for micropropagule formation in biocontrol fungi, Trichoderma asperellum and T. harzianum

  • 1.

    Department of Biology, Faculty of Science, Universiti Putra Malaysia, 43400 Serdang Selangor, Malaysia

  • 2.

    Department of Botany, Faculty of Science, Omar Al Mukhtar University, Al Bayda, Libya

  • 3.

    Department of Microbiology, Faculty of Biotechnology and Biomolecular Sciences, Universiti Putra Malaysia, 43400 Serdang, Selangor, Malaysia

  • Received Date: 27 March 2020
  • Revised Date: 17 May 2020
  • Published Online: 03 June 2020
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Studies in Fungi  5,  Article number: 9  (2020)  |  Cite this article

Abstract: Trichoderma species have shown efficiency on biocontrol of phytopathogens. For commercial application, it must be propagated in mass scale using a cost-effective method. As an alternative way to effectively deliver biocontrol fungi inoculum to the field; seven agro-based wastes including rice bran, biochar, empty fruit bunches, coconut fibre, compost, topsoil and mixed soil were used in this study for evaluating mass multiplication of Trichoderma species. Based on the evaluation of colony-forming units (cfu) among the agro-based waste media used, coconut fibre is the most suitable in promoting the sporulation of Trichoderma asperellum and T. harzianum. Trichoderma asperellum C1667 showed the higher micropropagule count through incubation period compared to T. harzianum C1675. After 120 days on the agro-based waste media, T. asperellum C1667 and T. harzianum C1675 produced the highest (7.717×105 cfu/g and 6.836±13.79×105 cfu/g) coconut fibres, respectively. Meanwhile, the mixed soil appeared with the lowest cfu. Coconut fibres were shown as a great biocomposting medium for both Trichoderma species. Findings of the present study are valuable for disease management using agro-based wastes as a cost-effective medium for biocontrol agents like Trichoderma species.

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    Cite this article
    ZAE Hasan, NAI Mohd Zainudin, A Aris, MH Ibrahim, MT Yusof. 2020. Evaluation of agro-based waste substrates for micropropagule formation in biocontrol fungi, Trichoderma asperellum and T. harzianum. Studies in Fungi 5(1):94-102 doi: 10.5943/sif/5/1/9
    ZAE Hasan, NAI Mohd Zainudin, A Aris, MH Ibrahim, MT Yusof. 2020. Evaluation of agro-based waste substrates for micropropagule formation in biocontrol fungi, Trichoderma asperellum and T. harzianum. Studies in Fungi 5(1):94-102 doi: 10.5943/sif/5/1/9
    shu

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