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Figure 1. Maximum likelihood phylogenetic tree generated from combined ITS and β-tubulin sequence data of 131 Diatrypaceae taxa and 1240 sites. For each node maximum likelihood bootstrap support values (MLBS) are given first, followed by Bayesian posterior probabilities (BYPP). ML bootstrap support values ≥ 75% and BYPP ≥ 0.95 are indicated at the nodes. Lower values are indicated by a dash (-). Nodes that were not recovered are indicated by asterisks (*). The new isolate is in red bold font. Ex-type sequences are in black bold font. The tree is rooted to Kretzschmaria deusta (CBS 826.72) and Xylaria hypoxylon (CBS 122.620).
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Figure 2. Diatrypella macrospora (MFLU 19-2401, new record). a Stromata erupting from bark. b Longitudinal section through stroma. c Section through a perithecium. d Peridium. e Paraphyses. f-i Asci. j-l Ascospores. m Germinated ascospore. n, o Top and bottom view of culture. Scale bars: a = 2 mm, b = 500 μm, c = 100 μm, d = 50 μm, e-j = 20 μm, k-m = 5 μm.
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Species Strains GenBank accession numbers ITS β-tubulin Allocryptovalsa HVFIG02 HQ692573 HQ692524 cryptovalsoidea Allocryptovalsa HVFIG05 HQ692574 HQ692525 cryptovalsoidea Allocryptovalsa elaeidis MFLUCC 15-0707 MN308410 MN340296 Allocryptovalsa polysporaT MFLUCC 17-0364 MF959500 MG334556 Allocryptovalsa rabenhorstii WA08CB HQ692619 HQ692523 Allocryptovalsa rabenhorstii WA07CO HQ692620 HQ692522 Allodiatrype arengaeT MFLUCC 15-0713 MN308411 MN340297 Allodiatrype elaeidicola MFLUCC 15-0737a MN308415 MN340299 Allodiatrype elaeidicola MFLUCC 15-0737b MN308416 - Allodiatrype elaeidis MFLUCC 15-0708a MN308412 MN340298 Allodiatrype elaeidis MFLUCC 15-0708b MN308413 - Allodiatrype thailandica MFLUCC 14-1210 KU315392 - Allodiatrype thailandica MFLUCC 15-0711 MN308414 - Anthostoma decipiensT IPV-FW349 AM399021 - Anthostoma decipiensT JL567 JN975370 JN975407 Cryptosphaeria eunomiaT C1C, CBS 216.87 AJ302417 - Cryptosphaeria eunomiaT C5C, CBS 223.87 AJ302421 - Cryptosphaeria ligniota CBS 273.87 KT425233 KT425168 Cryptosphaeria moravica/ CBS 244.87 HM164735 HM164769 Eutypa petrakii Cryptosphaeria pullmanensis ATCC 52655 KT425235 KT425170 Cryptosphaeria pullmanensis HBPF24 KT425202 GQ294014 Cryptosphaeria subcutanea CBS 240.87 KT425232 KT425167 Cryptosphaeria subcutanea DSUB100A KT425189 KT425124 Cryptovalsa ampelina A001 GQ293901 GQ293972 Cryptovalsa ampelina DRO101 GQ293902 GQ293982 Diatrype brunneospora CNP01 HM581946 HQ692478 Diatrype bullata UCDDCh400 DQ006946 DQ007002 Diatrype bullata D6C, CBS 215.87 AJ302422 - Diatrype decorticata 1056 KU320621 - Diatrype disciformisT D21C, CBS 205.87 AJ302437 - Diatrype disciformisT IRAN 2347C KR605644 KY352434 Diatrype enteroxantha HUEFS155114 KM396617 KT003700 Diatrype enteroxantha HUEFS155116 KM396618 KT022236 Diatrype macowaniana D15C, CBS 214.87 AJ302431 - Diatrype mangrovei MFLUCC 17-0412 MH304407 - Diatrype mangrovei MFLUCC 17-0391 MH304408 - Diatrype mangrovei MFLUCC 17-0394 MH304409 - Diatrype oregonensis CA117 GQ293934 GQ293996 Diatrype oregonensis DPL200 GQ293940 GQ293999 Diatrype palmicola MFLUCC 11-0018 KP744439 - Diatrype palmicola MFLUCC 11-0020 KP744438 - Diatrype polycocca D16C, CBS 213.87 AJ302432 - Diatrype prominens ATCC: MYA-4410 FJ430594 - Diatrype prominens SBen212 KU721868 - Diatrype sp. H2/4b MG020309 - Diatrype sp. H3/2b MG020294 - Diatrype sp. H2/5c MG020292 - Diatrype spilomea D17C AJ302433 - Diatrype stigma DCASH200 GQ293947 GQ294003 Diatrype stigma UCD23-Oe JX515704 JX515670 Diatrype undulata D20C, CBS 271.87 AJ302436 - Diatrype undulata Olrim324 AY354239 - Diatrype whitmanensis CDB011 GQ293954 GQ294010 Diatrype whitmanensis DCHES100 GQ293951 GQ294008 Diatrypella atlantica HUEFS 136873 KM396614 KR259647 Diatrypella atlantica HUEFS 194228 KM396615 KR363998 Diatrypella banksiae CPC 29118 KY173402 - Diatrypella banksiae CPC 29054 KY173401 - Diatrypella cephalanthi CBS 161.32 MH855258 - Diatrypella delonicis MFLUCC 15-1014 MH812994 MH847790 Diatrypella delonicis MFLU 16-1032 MH812995 MH847791 Diatrypella elaeidis MFLUCC 15-0279 MN308417 MN340300 Diatrypella favacea Isolate 380 KU320616 - Diatrypella favacea CBS 198.49 MH856491 - Diatrypella frostii UFMGCB 1917 HQ377280 - Diatrypella heveae MFLUCC 17-0368 MF959501 MG334557 Diatrypella heveae MFLUCC 15-0274 MN308418 MN340301 Diatrypella iranensis IRAN 2280C KDQ18 KM245033 KY352429 Diatrypella macrospora IRAN 2344C KDQ15 KR605648 KY352430 Diatrypella macrospora MFLUCC 21-0010 MW647094 MW677962 Diatrypella major Isolate 1058 KU320613 - Diatrypella prominens DL28A, ATCC 64182 AJ302442 - Diatrypella pulvinata H048 FR715523 FR715495 Diatrypella quercina DL30M AJ302444 - Diatrypella quercina CBS 108.18 MH854666 - Diatrypella sp. C6 KX611072 - Diatrypella sp. ENQ55 KX828138 KY352431 Diatrypella sp. MNQ75B KX828158 KY352432 Diatrypella tectonae MFLUCC 12-0172a KY283084 - Diatrypella tectonae MFLUCC 12-0172b KY283085 KY421043 Diatrypella verruciformisT UCROK1467 JX144793 JX174093 Diatrypella verruciformisT UCROK754 JX144783 JX174083 Diatrypella vulgaris HVFRA02 HQ692591 HQ692503 Diatrypella vulgaris HVGRF03 HQ692590 HQ692502 Diatrypella yunnanensis VT01 MN653008 - Eutypa armeniacae ATCC 28120 DQ006948 DQ006975 Eutypa astroidea E49C, CBS 292.87 AJ302458 DQ006966 Eutypa flavovirens E48C, CBS 272.87 AJ302457 DQ006959 Eutypa laevata E40C CBS 291.87 AJ302449 - Eutypa lataT CBS 290.87 HM164736 HM164770 Eutypa lataT EP18 HQ692611 HQ692501 Eutypa lataT RGA01 HQ692614 HQ692497 Eutypa lejoplaca CBS 248.87 DQ006922 DQ006974 Eutypa leptoplaca CBS 287.87 DQ006924 DQ006961 Eutypa maura CBS 219.87 DQ006926 DQ006967 Eutypa microasca BAFC 51550 KF964566 KF964572 Eutypa sparsa 3802 3b AY684220 AY684201 Eutypella cerviculataT EL59C AJ302468 - Eutypella cerviculataT M68 JF340269 - Eutypella leprosa EL54C, CBS 276.87 AJ302463 - Eutypella leprosa Isolate 60 KU320622 - Eutypella microtheca ADEL200 HQ692559 HQ692527 Eutypella microtheca BCMX01 KC405563 KC405560 Eutypella parasitica CBS 210.39 DQ118966 - Eutypella semicircularis MP4669 JQ517314 - Halodiatrype avicenniae MFLUCC 15-0953 KX573916 KX573931 Halodiatrype salinicolaT MFLUCC 15-1277 KX573915 KX573932 Kretzschmaria deusta CBS 826.72 KU683767 KU684190 Monosporascus cannonballusT CMM3646 JX971617 - Monosporascus ATCC 26931 FJ430598 - cannonballusT Neoeutypella baoshanensisT EL51C, CBS 274.87 AJ302460 - Neoeutypella baoshanensisT HMAS 255436 NR_164038 MH822888 Paraeutypella citricola HVGRF01 HQ692579 HQ692512 Paraeutypella citricola HVVIT07 HQ692589 HQ692521 Paraeutypella vitis UCD2291AR HQ288224 HQ288303 Paraeutypella vitis UCD2428TX FJ790851 GU294726 Pedumispora rhizophoraeT BCC44877 KJ888853 - Pedumispora rhizophoraeT BCC44878 KJ888854 - Peroneutypa alsophila EL58C, CBS 250.87 AJ302467 - Peroneutypa comosa BAFC 393 KF964568 - Peroneutypa curvispora HUEFS 136877 KM396641 - Peroneutypa diminutiasca MFLUCC 17-2144 MG873479 - Peroneutypa diminutispora HUEFS 192196 KM396647 - Peroneutypa kochiana EL53M AJ302462 - Peroneutypa longiasca MFLUCC 17-0371 MF959502 MG334558 Peroneutypa mackenziei MFLUCC 16-0072 KY283083 KY706363 Peroneutypa mangrovei NFCCI-4246 MG844286 MH094409 Peroneutypa rubiformis MFLUCC 17-2142 MG873477 - Peroneutypa scoparia DFMAL100 GQ293962 GQ294029 Peroneutypa scoparia IRAN 2345C KR605646 KY352452 Quaternaria quaternata CBS 278.87 AJ302469 - Quaternaria quaternata IRAN 2348C KR605645 KY352464 Xylaria hypoxylonT CBS 122.620 AM993141 KX271279 Table 1. GenBank accession numbers for the strains used in this study. The newly isolated strain of Diatrypella macrospora, is shaded. The type species of each genus is indicated as T and ex-type strains are in bold
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Species Shape of stromata Entostroma colour Perithecial neck (μm) Asci Ascospore Shape, colour, length × width (µm) Reference Shape Number of spores Length (p. sp. µm) Width (µm) Diatrype bullata (representative strain) ovoid white short neck cylindrical 8-spored 25-35 5-7 allantoid, pale yellow, 5-7.5 × 1.2 Rappaz (1987), Vasilyeva & Ma (2014) Diatrype disciformis (reference specimen) orbicular, disc-like yellowish white - cylindrical 8-spored 30-40 5-6 allantoid, pale yellow, 5-9× 1.5-2 Senanayake et al. (2015) Diatrype spilomea (representative strain) effuse white short neck - 8-spored 20-30 3-6 allantoid, pale yellow, 4.5-7 × 1-1.2 Rappaz (1987) Diatrype stigma (representative strain) effuse white short neck cylindrical 8-spored 25-50 5-6 allantoid, pale yellow, 5.8-10.5 × 1.2-2 Rappaz (1987), Vasilyeva & Ma (2014) Diatrype undulata (representative strain) effuse white short neck cylindrical 8-spored 25-40 4-7 allantoid, pale yellow, 5-8 × 1.2-1.8 Rappaz (1987), Vasilyeva & Ma (2014) Diatrypella hevea (Holotype) rounded to irregular white - clavate to cylindric-clavate multispored 80-113 10-21 hyaline to pale yellowish to pale brown oblong to allantoid, aseptate, slightly curved, 5–9 × 1–3 Senwanna et al. (2017) Diatrypella iranensis (Holotype) circular to ovoid whitish yellow - elongate, subcylindrical to clavate multispored - 6-9 allantoid, subhyaline 6-7 × 1-1.3 Mehrabi et al. (2015) Diatrypella macrospora (Holotype) circular white to yellow to light brown relatively long neck, 200–500, converge together elongate, more or less cylindrical 8-spored 110-150 10-15 allantoid, subhyaline 12-20 × 1.7-3 Mehrabi et al. (2016) Diatrypella macrospora (representative strain) discoid to irregular in shape white to, yellow to brown short neck, 110–350, converge together elongate, cylindrical to clavate multispored 72-120 8-14.5 allantoid, strongly curved, subhyaline 7.5–12 × 2–3.1 This study Diatrypella quercina (representative strain) Subregula rrounded or angular - - cylindrical to clavate multispored 80-120 10-12 allantoid, strongly curved 8–12 × 2–3 Croxall (1950), Saccardo (1882) Diatrypella tectonea circular to irregular white to short neck clavate multispored 100-128 15.5-21.5 yellowish to brown, ellipsoidal to cylindrical or elongate-allantoid, 7–9 × 2–2.5 Shang et al. (2017) Diatrypella verruciformis (representative strain) circular, subconical white -
converge togetherspindle-shaped multispored 100-132 11-11.5 allantoid, moderately or variously curved subolivaceous 6–8 × 1–2 Glawe & Rogers (1984) Table 2. Comparative morphology of Diatrypella macrospora and its relative species
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