Figures (6)  Tables (5)

    • Figure 1. 

      Flow chart showing the step-by-step procedure done in the evaluation of optimum culture conditions for the mycelial growth of Xylaria papulis Lloyd.

    • Figure 2. 

      Mycelia of X. papulis Lloyd produced from (a) Corn Grit Broth, (b) Rice Bran Broth, (c) Potato Sucrose Broth (sprouting young fruiting bodies encircled), and (d) Coconut Water.

    • Figure 3. 

      Mycelia of X. papulis Lloyd produced from different pH levels of PSB such as (a) pH 8.5, (b) pH 8.0, (c) pH 7.5, (d) pH 7.0, (e) pH 6.5, (f) pH 6.0, (g) pH 5.0, and (h) pH 5.5 after 10 d of incubation.

    • Figure 4. 

      Mycelial biomass of X. papulis Lloyd produced after 10 d from shaking condition at (a) 70 rpm and (b) static condition.

    • Figure 5. 

      Mycelia of X. papulis Lloyd produced after 10 d of incubation at different temperature conditions such as (a) refrigerated at 10 °C, (b) airconditioned at 23 °C, and (c) room temperature at 28 °C.

    • Figure 6. 

      Mycelia of X. papulis Lloyd produced after incubation at different illumination conditions such as (a) continued lighting, (b) alternating light and dark, and (c) totally dark for 10 d. Encircled in red are fruiting bodies observed in continued lighting condition.

    • Culture mediaMycelial thickness*
      (mm)      		Dry mycelial weight*
      (mg)
      Coconut Water (CW)1.02 ± 0.40c315.00 ± 22.60b
      Corn Grit Broth (CGB)1.42 ± 0.12bc88.00 ± 55.40c
      Rice Bran Broth (RBB)1.70 ± 0.51ab345.10 ± 24.70b
      Potato Sucrose Broth (PSB)2.31 ± 0.22a814.80 ± 57.60a
      * Values are mean ± SD. Means in a column that do not share a letter are significantly different at a 5% level of significance using Tukey's Comparison of Means.

      Table 1. 

      Mycelial growth performance of X. papulis Lloyd after 10 d of incubation in different indigenous liquid culture media.

    • pH level (PSB)Mycelial thickness* (mm)Dry mycelial eeight* (mg)
      5.01.51 ± 0.38bc431.20 ± 26.50c
      5.51.68 ± 0.43b584.00 ± 57.30ab
      6.02.53 ± 0.44a651.10 ± 26.40a
      6.51.96 ± 0.63ab676.10 ± 39.10a
      7.01.79 ± 0.67b506.00 ± 76.00bc
      7.51.78 ± 0.50b458.00 ± 10.77c
      8.01.57 ± 0.43bc414.30 ± 21.30c
      8.51.07 ± 0.52c242.52 ± 62.40d
      * Values are mean ± SD. Means in a column that do not share a letter are significantly different at a 5% level of significance using Tukey's Comparison of Means.

      Table 2. 

      Mycelial growth performance of X. papulis Lloyd in optimum liquid medium (PSB) at different pH levels.

    • Agitation conditionMycelial thickness* (mm)Dry mycelial weight* (mg)
      Shaking (70 rpm)0.93 ± 0.45b233.90 ± 53.4b
      Static1.94 ± 0.72a381.50 ± 65.0a
      * Values are mean ± SD. Means in a column that do not share a letter are significantly different at 5% level of significance using t-test.

      Table 3. 

      Mycelial growth performance of X. papulis Lloyd on different agitation conditions after 10 d of incubation.

    • Temperature conditionMycelial thickness* (mm)Dry mycelial weight* (mg)
      Refrigerated (10 °C)0.00 ± 0.00b0.00 ± 0.00b
      Airconditioned (23 °C)1.80 ± 0.54a318.00 ± 47.60a
      Room temperature (28 °C)1.99 ± 0.79a320.00 ± 36.70a
      * Values are mean ± SD. Means in a column that do not share a letter are significantly different at 5% level of significance using Tukey's Comparison of Means.

      Table 4. 

      Mycelial growth performance of X. papulis Lloyd in different temperature conditions after 10 d of incubation.

    • Illumination conditionMycelial thickness* (mm)Dry mycelial weight* (mg)
      Lit1.99 ± 0.41a405.20 ± 57.30a
      Alternate light and dark (every 12 h)1.25 ± 0.50b380.80 ± 20.80a
      Dark1.17 ± 0.48b403.70 ± 13.70a
      *Values are mean ± SD. Means in a column that do not share a letter are significantly different at 5% level of significance using Tukey's Comparison of Means.

      Table 5. 

      Mycelial growth performance of X. papulis Lloyd in different illumination conditions after 10 d of incubation.