Study on the mutagenic effects in the soybean genome accelerates breeding

Tiyun Liang; Miaohua He; Rui Xu; Qingyu Wang; Jianyuan Chen; Lin Yang; Yu Xia; Xuan Luo; Mengyuan Zhu; Wei Li; Jie Ren; Zhiqiang Xia; Meiling Zou; Tiyun Liang; Miaohua He; Rui Xu; Qingyu Wang; Jianyuan Chen; Lin Yang; Yu Xia; Xuan Luo; Mengyuan Zhu; Wei Li; Jie Ren; Zhiqiang Xia; Meiling Zou

doi:10.48130/tp-0025-0003

Figures (4) Tables (2)

Figure 1.
Impact of different radiation doses on the growth of soybeans. (a) Size of leaves at each dose. (b) Plant height at each dose. (c) Statistics of lethality at each dose level. (d) Average plant height at each dose level. TL180 refers to the Tianlong No.1 soybean population at 180 Gy, TL200 refers to the Tianlong No.1 soybean population at 200 Gy, and TL220 refers to the Tianlong No.1 soybean population at 220 Gy.
Figure 2.
Distribution of genomic mutations induced by an electron accelerator at various dose levels. TL180 represents the Tianlong No.1 soybean population at 180 Gy, TL200 represents the Tianlong No.1 soybean population at 200 Gy, W180 represents the Williams 82 population at 180 Gy, and W200 represents the Williams 82 population at 200 Gy.
Figure 3.
Distribution of genomic mutations induced by an electron accelerator in the M1 population at each dose level. (a) Proportion of SNPs, INSs, and DELs in different dose treatment groups. (b) Average number of mutations induced in different dose treatment groups. (c) Average mutation frequency in different dose treatment groups. (d) Mutation frequency in individual samples induced at each dose level.
Figure 4.
Comprehensive analysis of mutations induced by various dose treatment groups. (a) Distribution of mutations in gene structure across different doses. (b) Ts/Tv ratio at various doses. (c) Average number of genes affected by mutations at different doses. (d) Total number of amino acid alterations induced at various doses.

Sample	Number	Total loci	Variant loci	Mutation frequency %	Average SNPs	Average insertions	Average deletions
TL180	59	55,938,993	109,878	0.1964	1676.86	98.64	86.83
W180	64	75,979,433	167,151	0.2200	2339.14	155.00	117.59
TL200	86	123,112,699	212,685	0.1728	2161.94	164.87	146.27
W200	85	163,648,886	512,890	0.3134	5144.12	441.55	448.33

Table 1.

Soybean M1 generation mutation information statistics.

Sample	High	Low	Moderate	Modifier	Total
TL180	2,800	9,138	16,249	206,267	234,454
W180	4,563	14,274	25,191	327,616	371,644
TL200	6,812	17,991	32,613	408,042	465,458
W200	19,061	42,268	78,798	960,523	1,100,650

Table 2.

The number of mutated genes induced under different dose treatment groups.