Figures (6)  Tables (3)

    • Figure 1. 

      Venn diagram of OTUs in CFF and CFR groups.

    • Figure 2. 

      Analysis of fungal community composition of seven CF samples. (a) Phylum level; (b) class level; (c) order level; (d) family level; (e) genus level.

    • Figure 3. 

      Analysis of fungal community composition of the CFR samples. (a) Phylum level; (b) class level; (c) order level; (d) family level.

    • Figure 4. 

      Differences of fungal community at the genus level in (a) the CFR samples and (b) the CFF samples.

    • Figure 5. 

      Analysis of fungal community composition in the CFF samples. (a) Phylum level; (b) class level; (c) order level; (d) family level.

    • Figure 6. 

      Comparison of the differences of fungal community at the genus level between samples stored at RT and 4 °C for (a) 8-Week; (b) 16-Week; (c) 24-Week.

    • Storage time Storage temperature
      Room temperature 4 °C
      0-Week CF0W CF0W
      8-Week CFR8W CFF8W
      16-Week CFR16W CFF16W
      24-Week CFR24W CFF24W

      Table 1. 

      Sample information for the CF investigated in this study.

    • Sample Observed
      OTU numbers      		 Shannon Simpson Chao Coverage Genbank
      accession No.
      CF0W 81 2.45 0.18 81.00 1.000000 SAMN18864584
      CFR8W 126 2.63 0.12 129.33 0.999928 SAMN20297327
      CFR16W 51 1.01 0.67 51.00 0.999980 SAMN20297328
      CFR24W 38 2.95 0.08 38.00 1.000000 SAMN20297329
      CFF8W 128 1.02 0.62 129.62 0.999902 SAMN20297324
      CFF16W 196 2.00 0.25 208.00 0.999627 SAMN20297325
      CFF24W 131 3.29 0.08 136.00 0.999859 SAMN20297326

      Table 2. 

      Observed OTU numbers and alpha diversity indices of CF samples.

    • Sample No. The relative abundances of potentially toxigenic fungi in CF samples
      Rhizopus microsporus Penicillium oxalicum Trichothecium roseum Candida tropicalis Penicillium brevicompactum Beauveria bassiana Talaromyces rugulosus
      CF0W — — 47 18.29% 139 6.86% — — — — — — — —
      CFR8W — — 59 22.96% 422 20.82% — — 29 46.03% 16 66.67% 164 99.39%
      CFR16W — — — — 68 3.35% — — — — — — — —
      CFR24W — — — — 218 10.75% 448 100.00% — — — — — —
      CFF8W — — 12 4.67% 96 4.74% — — 26 41.27% — — — —
      CFF16W 3 100.00% 89 34.63% 112 5.53% — — 8 12.70% 8 33.33% 1 0.61%
      CFF24W — — 50 19.46% 972 47.95% — — — — — — — —
      Total 3 100.00% 257 100.00% 2027 100.00% 448 100.00% 63 100.00% 24 100.00% 165 100.00%

      Table 3. 

      Proportion of potentially toxigenic fungi in seven CF samples.