Figures (4)  Tables (4)

    • Figure 1. 

      Effects of 6-BA and NAA on clumping bud induction after, Y1: 0.1 mg·L−1 NAA + 1 mg·L−1 6-BA, Y2: 0.1 mg·L−1 NAA + 2 mg·L−1 6-BA, Y3: 0.1 mg·L−1 NAA + 3 mg·L−1 6-BA, Y4: 0.5 mg·L−1 NAA + 1 mg·L−1 6-BA,Y5: 0.5 mg·L−1 NAA + 2 mg·L−1 6-BA, Y6: 0.5 mg·L−1 NAA + 3 mg·L−1 6-BA,Y7: 1 mg·L−1 NAA + 1 mg·L−1 6-BA, Y8: 1 mg·L−1 NAA + 2 mg·L−1 6-BA, Y9: 1 mg·L−1 NAA + 3 mg·L−1 6-BA, the culture conditions are: light intensity at 2,000 lx, a 12-h light/dark cycle, a temperature of 25 ± 2 °C, and relative humidity of 50%−70%. Scale bars = 1 cm.

    • Figure 2. 

      Proliferation of clustered shoots (scale bars = 1 cm), CK: 0.00 mg·L−1 IBA, Z1: 0.10 mg·L−1 IBA, Z2: 0.20 mg·L−1 IBA, Z3: 0.30 mg·L−1 IBA, Z4: 0.40 mg·L−1 IBA, Z5: 0.50 mg·L−1 IBA, the culture conditions are: light intensity at 2,000 lx, a 12-h light/dark cycle, a temperature of 25 ± 2 °C, and relative humidity of 50%−70%.

    • Figure 3. 

      Thirty days of plant growth and rooting , the starting material was to cut about 3 cm from the tip of the shoot of the clumping buds cultured on the optimal proliferation medium for two generations to be used as an explant insertion into the rooting medium, G1: 0.00 mg·L−1 NAA, G2: 0.05 mg·L−1 NAA, G3: 0.10 mg·L−1 NAA, G4: 0.20 mg·L−1 NAA, the culture conditions are: light intensity at 2,000 lx, a 12-h light/dark cycle, a temperature of 25 ± 2 °C, and relative humidity of 50%−70%, scale bars = 1 cm.

    • Figure 4. 

      Tissue culture seedlings refining situation (scale bars = 1 cm). (a) Tissue culture seedlings. (b) The growth of seedlings after refining.

    • Treatment 75% alcohol (min) 3% sodium hypochlorite
      (min)      		 Contamination rate (%) Germination rate (%)
      T1 1 10 92.22 ± 5.09a 0.00 ± 0.00e
      T2 1 12 55.56 ± 5.09b 4.44 ± 3.85e
      T3 1 15 33.33 ± 3.33e 24.44 ± 3.85bc
      T4 2 10 50.00 ± 5.77b 5.56 ± 1.92e
      T5 2 12 24.44 ± 3.85cd 31.11 ± 5.09b
      T6 2 15 14.44 ± 1.92ef 50.00 ± 3.33a
      T7 3 10 46.67 ± 6.67b 24.44 ± 5.09bc
      T8 3 12 23.33 ± 3.33de 18.89 ± 1.92cd
      T9 3 15 10.00 ± 3.33f 16.67 ± 3.33d
      The data are mean ± standard deviation, and different letters after the same column of numbers indicate significant differences between different treatments (p < 0.05). Each treatment contains 30 explants, and each treatment was repeated three times.

      Table 1. 

      Statistics of disinfection contamination rate of 75% alcohol and 3% sodium hypochlorite.

    • Treatment NAA
      concentrations
      (mg·L−1)      		 6-BA
      concentrations
      (mg·L−1)      		 Induction
      rate (%)      		 Bud length
      (cm)
      Y1 0.1 1 38.89 ± 1.92d 0.96 ± 0.31a
      Y2 0.1 2 70.00 ± 3.33ab 0.69 ± 0.29b
      Y3 0.1 3 50.00 ± 3.33c 0.60 ± 0.18bc
      Y4 0.5 1 74.45 ± 6.94a 0.99 ± 0.34a
      Y5 0.5 2 71.11 ± 1.92ab 0.38 ± 0.10cd
      Y6 0.5 3 44.44 ± 5.09cd 0.28 ± 0.10d
      Y7 1 1 74.44 ± 5.09a 0.34 ± 0.08d
      Y8 1 2 71.11 ± 5.09ab 0.25 ± 0.11d
      Y9 1 3 60.00 ± 3.33b 0.31 ± 0.07d
      The data are mean ± standard deviation, and different letters after the same column of numbers indicate significant differences between different treatments (p < 0.05). Each treatment contains 30 explants, and each treatment was repeated three times.

      Table 2. 

      Effect of different hormone combinations on induction and multiplication of O. vulcanicola 'Sunset Velvet'.

    • Treatment IBA concentrations (mg·L−1) Biomass multiplication (folds) Browning rate (%)
      CK 0.00 20.32 ± 0.54a 18.65 ± 0.32d
      Z1 0.10 17.39 ± 0.70b 10.21 ± 0.33e
      Z2 0.20 14.99 ± 0.77c 17.98 ± 0.34d
      Z3 0.30 13.09 ± 0.38d 22.30 ± 0.46c
      Z4 0.40 11.78 ± 0.49d 26.88 ± 0.35b
      Z5 0.50 9.71 ± 0.59e 31.63 ± 0.19a
      The data are mean ± standard deviation, and different letters after the same column of numbers indicate significant differences between different treatments (p < 0.05). Each treatment contains 30 explants, and each treatment is repeated three times.

      Table 3. 

      Effect of different IBA concentrations on the proliferation of clustered shoots.

    • Treatment NAA concentrations (mg·L−1) Rooting rate (%) Number of roots Average root length (cm) Plant height (cm)
      G1 0.00 93.33 ± 3.33b 7.05 ± 0.10d 2.10 ± 0.49d 3.51 ± 0.06c
      G2 0.05 100.00 ± 0.00a 10.10 ± 0.44c 2.54 ± 0.04b 3.62 ± 0.08c
      G3 0.10 100.00 ± 0.00a 14.10 ± 0.10a 2.73 ± 0.15a 4.26 ± 0.05a
      G4 0.20 87.77 ± 1.93c 12.83 ± 0.25b 2.40 ± 0.10c 3.97 ± 0.11b
      The data are mean ± standard deviation, and different letters after the same column of numbers indicate significant differences between different treatments (p < 0.05). Each treatment contains 30 explants, and each treatment is repeated three times.

      Table 4. 

      Effects of different concentrations of NAA on plant growth and rooting.