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Figure 1. 

Detection of dipeptides with antihypertensive activity in deer meat extracts and HHP-treated deer meat sauce. Peaks corresponding to specific dipeptides are indicated. From top to bottom: (a) UV chromatogram at 280 nm, (b) total absorption chromatogram (TAC) obtained using a diode-array detector (DAD), and (c) the ion chromatogram at m/z = 227.1. Lines indicate peaks assigned to antihypertensive dipeptides. HHP treatment resulted in increased levels of these dipeptides compared with untreated deer meat extract. Details of the analytical conditions are provided in the materials and methods. These data represent typical examples from at least independently conducted experiments.

Figure 2. 

Lifespan of worms supplemented with deer meat extract and HHP-treated deer meat extract. The figure shows the survival curves for C. elegans across three groups: worms without supplementation (control, black line), worms supplemented with deer meat extract (green line), and worms supplemented with HHP-treated deer meat sauce (red line). The Kaplan-Meier survival curves for C. elegans (wild-type N2) at 20 °C are shown. The experiments were repeated in three independent experiments. Statistical significance was determined by the log-rank test (p < 0.05).

Figure 3. 

Migration distances of C. elegans under fatigue conditions with and without supplementation of HHP-treated deer meat sauce. Data are presented as mean ± SD. p < 0.05 indicates statistical significance between groups.