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Figure 1.
Key steps in the handmade cloning (HMC) process using ZP-free bovine oocytes. (a) Enucleation of a ZP-free oocyte by oriented bisection. (b) Cytoplasts positioned for fusion with a somatic donor cell (the arrow indicates the somatic cell placed between two cytoplasts). (c) Post-fusion reconstructed embryos undergoing parthenogenetic activation and initial development in vitro. (d) Blastocyst within a single well of the WOW culture system. (e) Expanded Day 6 blastocysts.
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Figure 2.
The cloned Huaxi beef calf was produced via vitrified HMC using ZP-free oocytes. Photographs taken shortly after birth (left) and nursing from the surrogate mother (right).
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