Figures (1)  Tables (1)

    • Figure 1. 

      Schematic representation of the paper-based preservation method for EPF.

    • Specie/strain Insect host/substrate Locality Year of
      preservationa      		 Time
      (years)b      		 Mycelial growth/
      sporulationc      		 Viability Pathogenicity
      (% sporulation)d
      Akanthomyces lecanii CEP054 Hemiptea: Aleyrodidae Buenos Aires 2018 3 C − −
      Akanthomyces muscarius CEP063 Hemiptera: Delphacidae Buenos Aires 2014 7 +++ 95 NT
      Akanthomyces muscarius CEP182 Hemiptera: Aphididae Buenos Aires 2015 6 +++ 100 NT
      Beauveria bassiana CEP317 Soil Buenos Aires 2012 9 +++ 70 95 (83)
      Beauveria bassiana CEP570 Hymenoptera: Formicidae Buenos Aires 2016 5 +++ 77 98 (95)
      Beauveria bassiana CEP588 Coleoptera Buenos Aires 2016 5 +++ 94 63 (77)
      Beauveria sp. CEP606 Hymenoptera: Formicidae Misiones 2017 4 +++ 99 100 (94)
      Cordyceps fumosorosea CEP307 Hemiptea: Aleyrodidae Buenos Aires 2018 3 + − −
      Cordyceps fumosorosea CEP371 Hemiptera: Aphididae Buenos Aires 2018 3 +++ 21 NT
      Cordyceps javanica CEP107 Hemiptera: Aleyrodidae Buenos Aires 2018 3 − − −
      Cordyceps sp. CEP708 Lepidoptera Jujuy 2018 3 − − −
      Lecanicillium aphanocladii CEP556 Araneae: Nemesiidae Buenos Aires 2018 3 +++ 100 NT
      Metarhizium anisopliae CEP399 Soil San Juan 2019 2 ++ 79 100 (85)
      Metarhizium anisopliae CEP404 Soil San Juan 2014 7 +++ 91 80 (75)
      Metarhizium hybridum CEP085e Hemiptera: Cercopidae Buenos Aires 2019 2 +++ 97 100 (100)
      Metarhizium hybridum CEP160e Hemiptera: Cercopidae Corrientes 2020 2 +++ 95 85 (65)
      Purpureocillium sp. CEP643 Hemiptera: Cicadidae Jujuy 2017 4 +++ 82 NT
      Purpureocillium sp. CEP780 Hemiptera: Pentatomidae Misiones 2018 3 +++ 35 NT
      a Year conidia were preserved in filter paper. b Time of preservation of conidia in filter paper (years). c Mycelial growth and sporulation. Normal mycelial growth and sporulation (+++). Scarce mycelial growth and sporulation (++). Normal mycelial growth, and there is no sporulation (+). No growth (−). Contamination by bacteria or fungi (C). Not tested with that species (NT). d Pathogenicity is expressed as virulence (% larval mortality); sporulation (%) of cadavers is shown in parentheses. e Metarhizium hybridum is a recently described species[11]. In this study, several isolates were identified as M. hybridum based on molecular data, and their corresponding GenBank accession numbers reported in the study by Lozano et al.[12].

      Table 1. 

      Evaluation of the quality of cultures, viability, pathogenicity, and purity after recovery from preservation on the filter paper method.