Figures (2)  Tables (2)

    • Figure 1. 

      Incidence of (a) young leaves in vitro, (b) young shoots in vitro, and (c) young plants in vivo.

    • Figure 2. 

      Comparative analysis of the same resource using different inoculation methods (the numbers 1 to 19 on the horizontal axis represented the resource codes in Table 1). Different letters (a, b, c) on the same group indicate values that were significantly different (p < 0.05) based on one-way ANOVA, Duncan post-hoc test.

    • Number Resource name Scientific name Resource type Origin
      1 Huazhen No. 5 Malus domestica Borkh. Rootstock variety Liaoning, China
      2 SH3 Malus domestica Borkh. Rootstock variety Shanxi, China
      3 SH6 Malus domestica Borkh. Rootstock variety Shanxi, China
      4 OT3 Malus domestica Borkh. Rootstock variety Canada
      5 M9T337 Malus domestica Borkh. Rootstock variety Netherlands
      6 B118 Malus domestica Borkh. Rootstock variety Russia
      7 E Shanjingzi No. 2 Malus baccata (L.) Borkh. Wild resources Russia
      8 Shajinhaitang Malus sargentii Rehd. Wild relatives Japan
      9 Xiaojinhaitang Malus xiaojinensis Cheng et Jiang Wild resources Sichuan, China
      10 Xiaogucheng Lenggunzi No. 1 Malus robusta (Carr.) Rehd. Landraces Hebei, China
      11 Daobazui Wuxianghaitang Malus honanensis Rehd. Wild resources Shanxi, China
      12 Longdonghaitang Malus kansuensis (Batal.) chneid. Wild resources Gansu, China
      13 Balenghaitang Malus robusta (Carr.) Rehd. Landraces Hebei, China
      14 Luanzhuang Shaguo Malus asiatica Nakai. Landraces Hebei, China
      15 Zhaojue Shanjingzi Malus baccata (L.) Borkh. Wild resources Yunnan, China
      16 Xishuhaitang Malus prattii (Hemsl.) Schneid. Wild resources Sichuan, China
      17 Chuisihaitang Malus halliana Koehne, Gatt.Pomac Wild resources Gansu, China
      18 Shidong Caiping No. 1 Malus domestica Borkh. Subsp. Chinensis Li Y.N. Landraces Hebei, China
      19 Yingyehaitang Malus ceracifolia Spach. Wild resources Liaoning, China

      Table 1. 

      Nineteen types of Malus plants.

    • Number Resource name Leaf inoculation Shoot inoculation Plant inoculation
      Disease index Coefficient of variation Resistance evaluation Disease index Coefficient of variation Resistance evaluation Disease index Coefficient of variation Resistance evaluation
      1 Huazhen No. 5 92.59a 13.85% HS 0.00c 0.00% HR 55.55b 0.00% MS
      2 SH3 92.59a 13.85% HS 0.00b 0.00% HR 87.30a 13.60% HS
      3 SH6 92.59a 13.85% HS 0.00c 0.00% HR 66.66b 19.24% S
      4 OT3 100a 0.00% HS 0.00c 0.00% HR 71.42b 29.59% S
      5 M9T337 77.78a 0.00% S 0.00b 0.00% HR 77.78a 18.07% S
      6 B118 92.59a 13.85% HS 92.59a 13.85% HS 74.60a 20.55% S
      7 E Shanjingzi No. 2 100 0.00% HS 0.00 0.00% HR 0.00 0.00% HR
      8 Shajinhaitang 80.00a 24.32% S 53.33a 36.48% MS 0.00b 0.00% HR
      9 Xiaojinhaitang 84.44a 21.67% HS 84.44a 17.76% HS 88.89a 24.30% HS
      10 Xiaogucheng Lenggunzi No. 1 77.78a 28.57% S 0.00b 0.00% HR 0.00b 0.00% HR
      11 Daobazui Wuxianghaitang 85.18a 15.06% HS 0.00c 0.00% HR 51.85b 17.49% MS
      12 Longdonghaitang 77.78a 35.63% S 17.78b 109.71% T 0.00b 0.00% HR
      13 Balenghaitang 77.78a 23.33% S 28.89b 48.65% T 40.00b 26.84% MS
      14 Luanzhuang Shaguo 45.56a 46.63% MS 8.89b 114.87% R 33.33a 0.00% T
      15 Zhaojue Shanjingzi 84.44a 21.67% HS 84.44a 21.67% HS 88.89a 13.18% HS
      16 Xishuhaitang 82.22a 21.32% HS 66.67b 23.57% MS 88.89a 17.68% HS
      17 Chuisihaitang 84.44a 21.67% HS 91.11a 12.60% HS 51.11b 27.50% MS
      18 Shidong Caiping No. 1 31.11a 52.70% T 16.67a 137.88% T 0.00b 0.00% HR
      19 Yingyehaitang 73.33a 23.90% S 4.44b 124.71% HR 0.00b 0.00% HR
      Different letters (a, b, c) on the same row indicated values that were significantly different (p < 0.05) based on one-way ANOVA, Duncan post-hoc test. And E Shanjingzi No. 2 exhibited zero variance across all inoculation methods, this extreme situation violated the basic assumptions of ANOVA. Therefore, no significance analysis was conducted on this set of data.

      Table 2. 

      Results of three different inoculation methods of Malus plants for resistance to fire blight.