Figures (2)  Tables (1)

    • Figure 1. 

      Functional characterization of AmGT28 and AmGT44. (a) Glycosylation of 1 by recombinant AmGT28/AmGT44 to produce 1a using UDP-Glc as the sugar donor. (b) UHPLC/UV chromatograms of the enzymatic reaction mixture. Control, reactions conducted using boiled protein; STD, reference standard. (c) MS and MS/MS spectra of 1a in the positive ion mode from the reaction catalyzed by AmGT28.

    • Figure 2. 

      Substrate specificity of AmGT28 and AmGT44. (a), (b) Conversion rates (%) of glycosylated products for substrates 1−15, using UDP-Glc as the sugar donor (n = 3). *, Products identified by comparing with reference standards; Δ, product purified from scaled-up reactions and characterized by NMR. (c) Structures of substrates 1−15.

    • Page no. on original PDF proof Line Original text Corrected text
      1 (Abstract) 3 medicarpin (1) (−)-astrapterocarpan (1)
      1 (Abstract) 3 medicarpin 3-O-glucoside (1a) (−)-astrapterocarpan 3-O-glucoside (1a)
      3 Left, 21 medicarpin (1) (−)-astrapterocarpan (1)
      3 Left, 24 medicarpin (1) (−)-astrapterocarpan (1)
      3 Left, 27 medicarpin 3-O-glucoside (1a) (−)-astrapterocarpan 3-O-glucoside (1a)