Figures (7)  Tables (1)

    • Figure 1. 

      The expression profiles of all transcripts from the low residual feed intake (LRFI) and high residual feed intake (HRFI) groups of Jian carp. (a) Heatmap of the transcripts' expression. (b) Volcano plot of the transcripts' expression.

    • Figure 2. 

      The GO signaling pathway annotation of differential alternative splicing genes (DASGs).

    • Figure 3. 

      The KEGG signaling pathway annotation of DASGs.

    • Figure 4. 

      The characteristics of the acsl4a and Hadha genes. (a) The exonic disparity of transcripts derived from acsl4a and Hadha. (b) The protein structural domains of different splice variants of acsl4a and Hadha. (c) The three-dimensional structure of Hadha_c and Hadha_d proteins.

    • Figure 5. 

      The expression profiles of transcripts derived from acsl4a (a) and Hadha (b) in the LRFI and HRFI groups.

    • Figure 6. 

      Quantitative real-time PCR (qRT-PCR) validation. (a) The expression profiles of 12 differentially expressed transcripts (DETs). (b) The relative expression levels of transcripts derived from acsl4a detected through qRT-PCR.

    • Figure 7. 

      Classification of SNP types. (a) The proportion of various SNP types among SNPs associated with DASGs. (b) The proportion of various SNP types among all SNPs.

    • Transcript ID Primer Sequence (5′–3′) Amplification size (bp)
      actn4_novel01 F TCAGTCTGGGTTACGACG 227
      R CTCACGCCTCAACTCCTC
      LOC109095800_novel01 F GGCGATAATGTGGACTTG 223
      R CCATCTAACTGCCAAAGC
      LOC109100422_novel05 F ATACGAGGATCGCAAGAG 203
      R GTTTCCGTGGAGGAGTAG
      LOC109105659_novel03 F CGGCATCTACGGCGTGTT 257
      R CCTTGCGTTTGCGTGTCT
      LOC109107915_novel04 F TGGCTTCCAAATCCTTAG 262
      R AGGTGCGGAGTCTGTGAT
      XM_042730762.1 F ACTGGTTGGGACTAAAGG 245
      R GCTGCGGTATTTCTTACA
      XM_042742207.1 F GCTTCCGATTGGTCAGTT 219
      R CGTGTCCATTAGGAGGTTT
      XM_042745279.1 F CTGATGAAATGATGACCCTA 255
      R CAGTAATGCTGGAGACCTT
      XM_042745964.1 F GCTCTGTATCGCCCACTC 275
      R CGTCACGGCTCTTCTTGT
      XM_042765333.1 F ACCCACTAAAGACTCCACA 190
      R ACCCGATAGACACTGACCT
      XM_042771159.1 F AATGGGCGATTCAGTAGCA 243
      R GGATGGTGGTGATGGTGG
      XM_042777294.1 F CTCGTGGCAAGCTGTCTA 228
      R GGAACTTAGGGTTACTCTGG
      acsl4a_all F CGTGGACAGGAAGAGCAT 142
      R AACAGCGAGGTCTGATGG
      acsl4a_b F TTGTTTCTTCGTTGCACATT 196
      R TGTACTGGGAACAAAAGAAC
      β-actin F CGTGATGGACTCTGGTGATG 162
      R TCGGCTGTGGTGGTGAAG

      Table 1. 

      Primer sequences of qRT-PCR.