Figures (4)  Tables (1)

    • Figure 1. 

      Phylogenetic placement of Agrocybe broadwayi from an ITS using IQ-TREE. SH-aLRT support (X ≥ 90%), aBayes support (Y ≥ 0.95), and ultrafast bootstrap support (Z ≥ 90%) are indicated above or below the branches as X/Y/Z.

    • Figure 2. 

      Phylogenetic placement of Xuaniella urbica among phylogenetically related species based on the ITS and LSU dataset using IQ-TREE. SH-aLRT support (X ≥ 90%), aBayes support (Y ≥ 0.95) and ultrafast bootstrap support (Z ≥ 90%) are indicated above or below the branches as X/Y/Z.

    • Figure 3. 

      Agrocybe broadwayi. (a)–(d) Basidiomata. (e), (f) Basidiospores. (g) Basidia. (h) Cheilocystidia. (i) Pleurocystidia. (j) Irregular elements. (k) Elements of pileipellis. (l) Elements on stipitipellis. Voucher specimens shown here: (a), (e), (g), (k) and (l) (TNS F-84789). (b), (f), (j) (TNS F-110973). (c) (TNS F-110974). Scale bars = 1 cm in (b); 2 cm in (c), (d); 3 cm in (a); 10 µm in (e)–(g), (i)–(l); 20 µm in (h).

    • Figure 4. 

      Xuaniella urbica. (a)–(d) Basidiomata. (e), (f) Basidiospores. (g), (h) Basidia. (i) Pileipellis. (j) Elements on stipitipellis. Voucher specimens shown here: (a) (TNS F-84784). (b), (f) (TNS F-84791). (c), (g) (TNS F-84752). (d), (e), (j) (TNS F-84786). (h), (i) (TNS F-84841). Scale bars = 1 cm in (c), (d); 2 cm in (a), (b); 5 µm in (e), (f); 10 µm in (g), (h), (j); 40 µm in (i).

    • Species1 Voucher and/or strain numbers ITS2 LSU2 Locality
      Agrocybe arvalis TENN 073105 MH615058 – USA
      Agrocybe arvalis DSM 9710 MN306191 – GERMANY
      Agrocybe broadwayi TNS F-84789 PX977843 – JAPAN: Ibaraki
      Agrocybe broadwayi TNS F-110973 PX977844 PX963668 JAPAN: Mie
      Agrocybe broadwayi TNS F-110974 PX977845 PX963669 JAPAN: Ibaraki
      Agrocybe broadwayi OMDL iNat # 224511333 PV818605 – USA
      Agrocybe broadwayi ABS1-BAGD PQ898389 – INDIA
      Agrocybe broadwayi (T) NY 814876 OP162979 – GRENADA
      Agrocybe cubensis (T) NY 814881 OP162978 – CUBA
      Agrocybe dura CBS:157.63 MH858248 – unknown
      Agrocybe dura CBS 246.38 MH855957 – USA
      Agrocybe eduradii (T) LE 313652 OM524381 – RUSSIA
      Agrocybe firma F26774 MZ314314 – USA
      Agrocybe firma RMA 17 MG663239 – USA
      Agrocybe imaii (T) SAPA:10000036 MZ725547 – JAPAN
      Agrocybe molesta MO502967 OR336123 – USA
      Agrocybe pediades M. van der Walt:VDW1454 MT304652 – SOUTH AFRICA
      Agrocybe pediades HFJAU0368 MN258622 – CHINA
      Agrocybe pediades LE 262824 JN684785 – RUSSIA
      Agrocybe praecox OS387 KC842389 – NORWAY
      Agrocybe praecox OMDL iNat # 225665840 PQ678613 – USA
      Agrocybe praecox HMAS 297971 OR236782 – CHINA
      Agrocybe praecox PDD 86836 KM975410 – NEW ZEALAND
      Agrocybe putaminum PDD 96108 KM975434 – NEW ZEALAND
      Agrocybe retigera FLAS:60923 MH016951 – USA
      Agrocybe retigera SL1223 OR527351 – SINGAPORE
      Agrocybe retigera FAFU04 MW362215 – CHINA
      Agrocybe retigera ISBW36 PQ331056 – PAKISTAN
      Agrocybe rivulosa CCB160 KF830098 – USA
      Agrocybe rivulosa KUBOT_KRMK_2020_95 MW487609 – INDIA
      Agrocybe smithii PBM3793 MG663269 – USA
      Agrocybe striatipes (T) HMAS286942 OQ186168 – CHINA
      Agrocybe subpediades LE 217898 JN684795 – RUSSIA
      Agrocybe vervacti M60 MW425942 – HUNGARY
      Agrocybe vervacti GC 95058 OQ845960 – ITALY
      Cyclocybe erebia ANGE318 KM260145 – ITALY
      Atheniella flavida FFAAS0355 MW969654 – CHINA
      Atheniella flavida (T) FFAAS0350 MW969653 MW969665 CHINA
      Atheniella flavoalba 604 JF908464 – ITALY
      Atheniella flavoalba CBS 258.53 MH857185 MH868723 FRANCE
      Atheniella flavoalba H6032608 MW540661 – FINLAND
      Atheniella flavoalba HMJAU43764 MT497547 – CHINA
      Clitocybula fuscostriata FFAAS1029 OR238881 OR238893 CHINA
      Clitocybula fuscostriata (T) FFAAS1030 OR238882 – CHINA
      Clitocybula intervenosa (T) BAP 613 MH414561 MH385335 SÃO TOMÉ E PRINCIPE
      Clitocybula oculus F26468 MZ317354 – USA
      Clitocybula oculus AFTOL-ID 1554 DQ192178 DQ151452 USA
      Delicatula integrella KA12-1305 KR673538 – KOREA
      Gerronema kuruvense HTBM3056 PX308920 PX309038 CHINA
      Hemimycena lactea OULU:GAJ15636 OR863440 OR863509 FINLAND
      Hydropodia silvae-nipponicae TNS F-82594 OQ676557 OQ676555 JAPAN: Ibaraki
      Hydropodia silvae-nipponicae (T) TNS F-82592 OQ676556 NG_242114 JAPAN: Ibaraki
      Trogia delicata DED 8235 (SFSU) MH414567 MH385341 SÃO TOMÉ E PRINCIPE
      Xuaniella urbica TNS F-84752 PX977846 PX963670 JAPAN: Ibaraki
      Xuaniella urbica TNS F-84784 PX977847 PX963671 JAPAN: Ibaraki
      Xuaniella urbica TNS F-84786 PX977848 PX963672 JAPAN: Ibaraki
      Xuaniella urbica TNS F-84791 PX977849 PX963673 JAPAN: Chiba
      Xuaniella urbica TNS F-84841 PX977850 PX963674 JAPAN: Kanagawa
      Xuaniella urbica HTBM2719 PX308955 PX309075 CHINA
      Xuaniella urbica HTBM2910 PX308939 PX309056 CHINA
      Xuaniella urbica (T) HKAS150761 PX308937 PX309053 CHINA
      Xeromphalina enigmatica (T) TENN-F-055726 KM066052 KM066079 FINLAND
      1 Holotype specimens are marked with (T). 2 GenBank accession numbers for each locus used in this study; PX963668 and PX963669 were excluded from the analyses. Bold indicates sequences obtained in this study.

      Table 1. 

      A list of specimens used in this study.