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Figure 1.
VuHSP60 genes' chromosomal positions.
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Figure 2.
Phylogenetic trees of the VuHSP60 gene family.
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Figure 3.
Conserved motifs, gene structure, conserved domains, and phylogenetic relationship of VuHSP60 proteins.
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Figure 4.
Predicted miRNA–VuHSP60 gene interaction network. Light blue circles represent VuHSP60 genes, yellow triangles represent miRNAs, and gray lines show predicted targeting interactions.
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Figure 5.
Synteny and collinearity analysis. Blue lines indicate the collinear HSP60 gene pairs within conserved syntenic blocks, while the faint gray lines in the background represent the genome-wide synteny (all other collinear gene pairs) in five species.
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Figure 6.
Cis-elements predicted in the promoter regions of VuHsp60 genes.
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Figure 7.
Expression levels of 25 VuHSP60 genes.
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Gene ID Gene name Chr aa MW pI Ins I Alip I GRAVY Localization Vigun01g052500.1 VuHSP60-1 Vu01 593 62.90 5.8 33.06 99.31 −0.102 Ch Vigun01g242300.1 VuHSP60-2 Vu01 535 59.15 5.61 39.99 97.53 −0.188 Cy Vigun03g007100.1 VuHSP60-3 Vu03 533 57.53 6.85 34.25 115.78 0.145 Cy Vigun03g090600.1 VuHSP60-4 Vu03 545 59.01 5.98 30.37 103.28 −0.043 Cy Vigun03g196200.1 VuHSP60-5.1 Vu03 588 62.61 7.66 30.72 105.19 0.071 Mi Vigun03g196200.2 VuHSP60-5.2 Vu03 485 51.67 6.08 26.04 108.82 0.105 Mi Vigun03g196200.3 VuHSP60-5.3 Vu03 476 50.57 6.08 25.75 109.85 0.112 Mi, Cy Vigun04g171700.1 VuHSP60-6 Vu04 555 60.31 5.97 39.26 101.8 −0.107 Cy Vigun05g005000.1 VuHSP60-7 Vu05 535 59.03 5.6 32.08 100.75 −0.158 Cy Vigun05g208400.1 VuHSP60-8 Vu05 527 57.16 5.55 35.11 101.48 −0.042 Cy Vigun06g042300.1 VuHSP60-9.1 Vu06 611 66.11 9.11 33.58 101.18 −0.084 Mi, Ch Vigun06g042300.3 VuHSP60-9.2 Vu06 539 58.20 8.81 31.42 102.56 −0.098 Ch Vigun07g050000.1 VuHSP60-10 Vu07 637 67.81 6.28 25.6 101.98 0.019 Mi Vigun07g166500.2 VuHSP60-11.1 Vu07 483 53.60 8.77 46.12 103.79 −0.17 Cy Vigun07g166500.4 VuHSP60-11.2 Vu07 461 50.53 5.73 38.82 104.53 −0.06 Cy Vigun07g166500.5 VuHSP60-11.3 Vu07 456 49.99 6.08 39.95 106.34 −0.083 Cy Vigun07g166500.7 VuHSP60-11.4 Vu07 436 47.96 5.73 37.34 105.16 −0.072 Cy Vigun07g185200.1 VuHSP60-12 Vu07 575 61.30 5.9 26.34 100.09 −0.087 Mi Vigun08g177100.1 VuHSP60-13 Vu08 560 60.31 6.2 35.37 96.04 −0.082 Cy Vigun10g173100.1 VuHSP60-14.1 Vu10 575 61.69 7.57 23.64 99.03 −0.142 Mi Vigun10g173100.2 VuHSP60-14.2 Vu10 467 50.51 5.2 27.35 104.18 −0.104 Ch, Cy Vigun11g107100.2 VuHSP60-15 Vu11 414 44.91 5.43 31.62 102.49 −0.015 Cy Vigun11g128900.1 VuHSP60-16 Vu11 527 57.10 5.45 34.04 102.03 −0.028 Cy Vigun11g139100.1 VuHSP60-17 Vu11 584 61.67 5.15 30.15 106.11 −0.026 Ch VigunL051601.1 VuHSP60-18 Contig_339 680 71.76 5.27 27.1 108.34 −0.021 Cy, Mi, Ch Note: Using TBtool II and CELLO, the following basic features of the VuHSP60 gene family members were predicted from the V. unguiculata genome. aa, amino acids; chr, chromosome; MW, molecular weight; pI, isoelectric point; Ins I, instability index; Alip I, aliphatic index; GRAVY, grand average hydropathicity; Cy, cytoplasm; Mi, mitochondria; Ch, chloroplast. Table 1.
Basic characteristics of the V. unguiculata HSP60 gene family.
Figures
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Tables
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