doi:10.48130/fia-0026-0045

[1]

Vancells Lujan P, Viñas Esmel E, Sacanella Meseguer E. 2021. Overview of non-alcoholic fatty liver disease (NAFLD) and the role of sugary food consumption and other dietary components in its development. Nutrients 13(5):1442

doi: 10.3390/nu13051442

[2]

Chen JF, Wu ZQ, Liu HS, Yan S, Wang YX, et al. 2024. Cumulative effects of excess high-normal alanine aminotransferase levels in relation to new-onset metabolic dysfunction-associated fatty liver disease in China. World Journal of Gastroenterology 30(10):1346−1357

doi: 10.3748/wjg.v30.i10.1346

[3]

Mitrovic B, Obradovic M, Gluvic Z, Radunovic M, Rizzo M, et al. 2022. Non-alcoholic fatty liver disease, metabolic syndrome, and type 2 diabetes mellitus: where do we stand today? Archives of Medical Science 19:884

doi: 10.5114/aoms/150639

[4]

Ruan S, Huang L, Song J, Yi Z, Sun W, et al. 2025. Global burden trends and forecasts for MAFLD in adolescents and young adults from 1990 to 2021. Scientific Reports 15:13534

doi: 10.1038/s41598-025-98489-9

[5]

Zhang H, Sham TT, Li C, Wang Y, Li CY, et al. 2023. Polygoni Cuspidati Rhizoma et Radix extract attenuates high-fat diet-induced NAFLD in rats: impact on untargeted serum metabolomics and liver lipidomics. Pharmacological Research–Modern Chinese Medicine 9:100335

doi: 10.1016/j.prmcm.2023.100335

[6]

Zhao Q, Wei M, Zhang S, Huang Z, Lu B, et al. 2020. The water extract of Sophorae tonkinensis Radix et Rhizoma alleviates non-alcoholic fatty liver disease and its mechanism. Phytomedicine 77:153270

doi: 10.1016/j.phymed.2020.153270

[7]

Liu D, Zhan J, Wang S, Chen L, Zhu Q, et al. 2024. Chrysanthemum morifolium attenuates metabolic and alcohol-associated liver disease via gut microbiota and PPARα/γ activation. Phytomedicine 130:155774

doi: 10.1016/j.phymed.2024.155774

[8]

Liu YF, Ling N, Zhang B, Chen C, Mo XN, et al. 2024. Flavonoid-Rich mulberry leaf extract modulate lipid metabolism, antioxidant capacity, and gut microbiota in high-fat diet-induced obesity: potential roles of FGF21 and SOCS₂. Food & Medicine Homology 1(2):9420016

doi: 10.26599/fmh.2024.9420016

[9]

Ghani I, An Y, Qiao Q, He S, Li Z. 2024. Polyphenols from foxtail millet improve non-alcoholic fatty liver disease by regulating intestinal microbiome in mice. Foods 13(11):1683

doi: 10.3390/foods13111683

[10]

Zhao Y, Zhang Y, Yang H, Xu Z, Li Z, et al. 2024. A comparative metabolomics analysis of phytochemicals and antioxidant activity between broccoli floret and by-products (leaves and stalks). Food Chemistry 443:138517

doi: 10.1016/j.foodchem.2024.138517

[11]

Salas-Millán JÁ, Mackrill J, Melgar S, Aguayo E. 2025. Lactofermented beverage from broccoli leaves induces calcium dysregulation and ER-mitochondrial dysfunction in colorectal cancer cells: a proteomic perspective. Food Research International 217:116819

doi: 10.1016/j.foodres.2025.116819

[12]

Li L, Ma P, Nirasawa S, Liu H. 2024. Formation, immunomodulatory activities, and enhancement of glucosinolates and sulforaphane in broccoli sprouts: a review for maximizing the health benefits to human. Critical Reviews in Food Science and Nutrition 64(20):7118−7148

doi: 10.1080/10408398.2023.2181311

[13]

Gasmi A, Gasmi Benahmed A, Shanaida M, Chirumbolo S, Menzel A, et al. 2024. Anticancer activity of broccoli, its organosulfur and polyphenolic compounds. Critical Reviews in Food Science and Nutrition 64(22):8054−8072

doi: 10.1080/10408398.2023.2195493

[14]

Llorach R, Espín JC, Tomás-Barberán FA, Ferreres F. 2003. Valorization of cauliflower (Brassica oleracea L. var. Botrytis) by-products as a source of antioxidant phenolics. Journal of Agricultural and Food Chemistry 51(8):2181−2187

doi: 10.1021/jf021056a

[15]

Fang J, Liu T, Wang Y, Kang SG, Huang K, et al. 2024. β-ionone prevents dextran sulfate sodium-induced ulcerative colitis and modulates gut microbiota in mice. Food Innovation and Advances 3(4):320−331

doi: 10.48130/fia-0024-0031

[16]

Vornoli A, Vizzarri F, Della Croce CM, Grande T, Palazzo M, et al. 2022. The hypolipidemic, anti-inflammatory and antioxidant effect of Kavolì® aqueous extract, a mixture of Brassica oleracea leaves, in a rat model of NAFLD. Food and Chemical Toxicology 167:113261

doi: 10.1016/j.fct.2022.113261

[17]

Zhou RY, Song Q, Hu H, Zeng SY, Tan XY, et al. 2025. Investigating therapeutic effects and the underlying mechanisms of Jia-Wei-Yin-Chen-Hao-Tang in non-alcoholic fatty liver disease based on network pharmacology analysis and experimental validation. Journal of Ethnopharmacology 352:120158

doi: 10.1016/j.jep.2025.120158

[18]

Li HY, Huang SY, Zhou DD, Xiong RG, Luo M, et al. 2023. Theabrownin inhibits obesity and non-alcoholic fatty liver disease in mice via serotonin-related signaling pathways and gut-liver axis. Journal of Advanced Research 52:59−72

doi: 10.1016/j.jare.2023.01.008

[19]

Zhao Y, Zhang W, Yang H, Xu Z, Wang X, et al. 2025. Effects of drying methods on phytochemicals and antioxidant activity of broccoli by-products. Food Research International 208:116284

doi: 10.1016/j.foodres.2025.116284

[20]

Jia W, Wang K, Zhang S, Lu W, Du A, et al. 2022. Integrating network pharmacology and in vivo experimental validation to reveal the alleviation of mailuoning oral liquid on non-alcoholic fatty liver disease. Phytomedicine 104:154326

doi: 10.1016/j.phymed.2022.154326

[21]

Ren QD, Li MR, Farag MA, Qiu LL, Wang YA, et al. 2026. Pomegranate peel extract alleviates diabetic retinopathy by suppressing the PI3K/AKT/HIF-1α/VEGF pathway and gut microbiota modulation. Journal of Advanced Research 85:137−154

doi: 10.1016/j.jare.2025.10.048

[22]

Lin LZ, Harnly JM. 2009. Identification of the phenolic components of collard greens, kale, and Chinese broccoli. Journal of Agricultural and Food Chemistry 57(16):7401−7408

doi: 10.1021/jf901121v

[23]

Gudiño I, Casquete R, Martín A, Wu Y, Benito MJ. 2024. Comprehensive analysis of bioactive compounds, functional properties, and applications of broccoli by-products. Foods 13(23):3918

doi: 10.3390/foods13233918

[24]

Shao Y, Jiang J, Ran L, Lu C, Wei C, et al. 2014. Analysis of flavonoids and hydroxycinnamic acid derivatives in rapeseeds (Brassica napus L. var. napus) by HPLC-PDA–ESI (–)-MSⁿ/HRMS. Journal of Agricultural and Food Chemistry 62(13):2935−2945

doi: 10.1021/jf404826u

[25]

Gould KS. 2004. Nature's Swiss army knife: the diverse protective roles of anthocyanins in leaves. Journal of Biomedicine & Biotechnology 2004(5):314−320

doi: 10.1155/S1110724304406147

[26]

Vallejo F, Tomás-Barberán FA, García-Viguera C. 2003. Phenolic compound contents in edible parts of broccoli inflorescences after domestic cooking. Journal of the Science of Food and Agriculture 83(14):1511−1516

doi: 10.1002/jsfa.1585

[27]

Nićiforović N, Abramovič H. 2014. Sinapic acid and its derivatives: natural sources and bioactivity. Comprehensive Reviews in Food Science and Food Safety 13(1):34−51

doi: 10.1111/1541-4337.12041

[28]

Chen AY, Chen YC. 2013. A review of the dietary flavonoid, kaempferol on human health and cancer chemoprevention. Food Chemistry 138(4):2099−2107

doi: 10.1016/j.foodchem.2012.11.139

[29]

Ullah H, Dacrema M, Buccato DG, Fayed MAA, de Lellis LF, et al. 2025. A narrative review on plant extracts for metabolic syndrome: efficacy, safety, and technological advances. Nutrients 17(5):877

doi: 10.3390/nu17050877

[30]

Martins T, Oliveira PA, Pires MJ, Neuparth MJ, Lanzarin G, et al. 2022. Effect of a sub-chronic oral exposure of broccoli (Brassica oleracea L. var. Italica) by-products flour on the physiological parameters of FVB/N mice: a pilot study. Foods 11(1):120

doi: 10.3390/foods11010120

[31]

Navarro-Hortal MD, Romero-Márquez JM, López-Bascón MA, Sánchez-González C, Xiao J, et al. 2024. In vitro and in vivo insights into a broccoli byproduct as a healthy ingredient for the management of Alzheimer's disease and aging through redox biology. Journal of Agricultural and Food Chemistry 72(10):5197−5211

doi: 10.1021/acs.jafc.3c05609

[32]

Zhou Y, Yang H, Li Y, Lynch B, Jia X. 2015. Broccoli seed extract: genotoxicity and subchronic toxicity studies. Regulatory Toxicology and Pharmacology 73(1):442−451

doi: 10.1016/j.yrtph.2015.08.003

[33]

Fabbrini E, Sullivan S, Klein S. 2010. Obesity and nonalcoholic fatty liver disease: biochemical, metabolic, and clinical implications. Hepatology 51(2):679−689

doi: 10.1002/hep.23280

[34]

Colosimo S, Mitra SK, Chaudhury T, Marchesini G. 2023. Insulin resistance and metabolic flexibility as drivers of liver and cardiac disease in T2DM. Diabetes Research and Clinical Practice 206:111016

doi: 10.1016/j.diabres.2023.111016

[35]

da Silva Pereira ENG, Franco RLC, Santos RDCD, Daliry A. 2025. Statins and non-alcoholic fatty liver disease: a concise review. Biomedicine & Pharmacotherapy 183:117805

doi: 10.1016/j.biopha.2024.117805

[36]

Wang GW, Zhang XL, Wu QH, Jin YB, Ning CT, et al. 2019. The hepatoprotective effects of Sedum sarmentosum extract and its isolated major constituent through Nrf2 activation and NF-κB inhibition. Phytomedicine 53:263−273

doi: 10.1016/j.phymed.2018.09.023

[37]

Hagberg CE, Spalding KL. 2024. White adipocyte dysfunction and obesity-associated pathologies in humans. Nature Reviews Molecular Cell Biology 25(4):270−289

doi: 10.1038/s41580-023-00680-1

[38]

Mirarchi A, Mare R, Musolino V, Nucera S, Mollace V, et al. 2022. Bergamot polyphenol extract reduces hepatocyte neutral fat by increasing beta-oxidation. Nutrients 14(16):3434

doi: 10.3390/nu14163434

[39]

Zhang X, Zhang J, Zhou Z, Xiong P, Cheng L, et al. 2024. Integrated network pharmacology, metabolomics, and transcriptomics of Huanglian-Hongqu herb pair in non-alcoholic fatty liver disease. Journal of Ethnopharmacology 325:117828

doi: 10.1016/j.jep.2024.117828

[40]

Huang X, Liu G, Guo J, Su Z. 2018. The PI3K/AKT pathway in obesity and type 2 diabetes. International Journal of Biological Sciences 14(11):1483−1496

doi: 10.7150/ijbs.27173

[41]

Alhamoud Y, Wu J, Ahmad MI, Chen F, Feng F, et al. 2023. 6-Gingerol induces browning of white adipose tissue to improve obesity through PI3K/AKT-RPS6-mediated thermogenesis pathway. Food Frontiers 4(3):1285−1297

doi: 10.1002/fft2.251

[42]

Liu D, Zhang Y, Liu Y, Hou L, Li S, et al. 2018. Berberine modulates gut microbiota and reduces insulin resistance via the TLR4 signaling pathway. Experimental and Clinical Endocrinology & Diabetes 126(8):513−520

doi: 10.1055/s-0043-125066

[43]

Feng Z, Pang L, Chen S, Pang X, Huang Y, et al. 2020. Didymin ameliorates dexamethasone-induced non-alcoholic fatty liver disease by inhibiting TLR4/NF-κB and PI3K/Akt pathways in C57BL/6J mice. International Immunopharmacology 88:107003

doi: 10.1016/j.intimp.2020.107003

[44]

Kong WJ, Vernieri C, Foiani M, Jiang JD. 2020. Berberine in the treatment of metabolism-related chronic diseases: a drug cloud (dCloud) effect to target multifactorial disorders. Pharmacology & Therapeutics 209:107496

doi: 10.1016/j.pharmthera.2020.107496

[45]

Martel J, Chang SH, Ko YF, Hwang TL, Young JD, et al. 2022. Gut barrier disruption and chronic disease. Trends in Endocrinology & Metabolism 33(4):247−265

doi: 10.1016/j.tem.2022.01.002

[46]

Tilg H, Adolph TE, Dudek M, Knolle P. 2021. Non-alcoholic fatty liver disease: the interplay between metabolism, microbes and immunity. Nature Metabolism 3(12):1596−1607

doi: 10.1038/s42255-021-00501-9

[47]

Ghannam RB, Techtmann SM. 2021. Machine learning applications in microbial ecology, human microbiome studies, and environmental monitoring. Computational and Structural Biotechnology Journal 19:1092−1107

doi: 10.1016/j.csbj.2021.01.028

[48]

Deng Y, Umbach AK, Neufeld JD. 2024. Nonparametric richness estimators Chao1 and ACE must not be used with amplicon sequence variant data. The ISME Journal 18:wrae106

doi: 10.1093/ismejo/wrae106

[49]

Lau HCH, Sung JJ, Yu J. 2021. Gut microbiota: impacts on gastrointestinal cancer immunotherapy. Gut Microbes 13(1):1869504

doi: 10.1080/19490976.2020.1869504

[50]

Zeng Q, Li D, He Y, Li Y, Yang Z, et al. 2019. Discrepant gut microbiota markers for the classification of obesity-related metabolic abnormalities. Scientific Reports 9(1):13424

doi: 10.1038/s41598-019-49462-w

[51]

Silva FP, De Miranda DA, Carnier M, Maza PK, Boldarine VT, et al. 2021. Low dose of Juçara pulp (Euterpe edulis Mart.) minimizes the colon inflammatory milieu promoted by hypercaloric and hyperlipidic diet in mice. Journal of Functional Foods 77:104343

doi: 10.1016/j.jff.2020.104343

[52]

Yang C, Xu Z, Deng Q, Huang Q, Wang X, et al. 2020. Beneficial effects of flaxseed polysaccharides on metabolic syndrome via gut microbiota in high-fat diet fed mice. Food Research International 131:108994

doi: 10.1016/j.foodres.2020.108994

[53]

Li X, Chen P, Zhang Y, Zhang J, Shen S, et al. 2023. Intervention time modified the effect of inulin on high-fat diet-induced obesity and gut microbial disorders. Food Frontiers 4(2):933−944

doi: 10.1002/fft2.243

[54]

Zhang C, Wang G, Yin X, Gou L, Guo M, et al. 2024. Hepatic protein phosphatase 1 regulatory subunit 3G alleviates obesity and liver steatosis by regulating the gut microbiota and bile acid metabolism. Journal of Pharmaceutical Analysis 14(8):100976

doi: 10.1016/j.jpha.2024.100976