Antimicrobial and cytotoxic activities of endophytic fungus Colletotrichum gloeosporioides isolated from endemic tree Cinnamomum malabatrum
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1.
Department of Botany, Virudhunagar Hindu Nadar Senthikumara Nadar College (Autonomous), Virudhunagar – 626 001, Tamil Nadu, India
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2.
Department of Ecology, French Institute of Pondicherry, Puducherry- 605 001
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3.
Department of Pharmaceutical Biotechnology/ Pharmacognosy, KMCH College of Pharmacy, Coimbatore – 641 048, Tamil Nadu, India
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Received:
12 March 2016
Revised:
17 May 2016
Published online:
19 June 2016
Studies in Fungi
1(1): 104−113(2016) |
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Abstract
In a survey of endophytic fungi associated with endemic plant Cinnamomum malabatrum leaves harbored a bioactive endophytic isolate CMS 3 was identified as Colletotrichum gloeosporioides through morphological and phylogenetic analysis based on ITS-rDNA. The ethyl acetate extract of fermentation broth of Colletotrichum gloeosporioides CMS 3 displayed antimicrobial activity against gram positive and gram negative bacteria as well as the fungal pathogen, Candida albicans. The ethyl acetate crude extract showed in vitro cytotoxicity against the HeLa, MCF-7 and MG63 cancer cell lines with the IC50 values of 94.2µg/ml, 84.3µg/ml and 162µg/ml respectively. Gas chromatography and Mass Spectrophotometry (GC-MS) analysis of crude extract confirmed that CMS 3 was a prolific producer of secondary metabolites, in which nearly 74% of the metabolites not listed in the NIST database. Major compounds were phenol 3, 5-dimethoxy acetate (11.82%), 4'-isopropylidene-bis-(2-cyclohexyl) phenol, NDidehydrohexacarboxyl-2, 4, 5-trimethylpiperazine and 1, 2, 4-Triazolium ylide. These metabolites may be responsible for its antimicrobial and cytotoxic activities.
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Copyright: © 2016 by the author(s). This article is an open access article distributed under Creative Commons Attribution License (CC BY 4.0), visit https://creativecommons.org/licenses/by/4.0/.
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R Packiaraj, S Jeyakumar, N Ayyappan, N Adhirajan, G Premkumar, K Rajarathinam, S Muthuramkumar. 2016. Antimicrobial and cytotoxic activities of endophytic fungus Colletotrichum gloeosporioides isolated from endemic tree Cinnamomum malabatrum. Studies in Fungi 1(1):104−113 doi: 10.5943/sif/1/1/10
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R Packiaraj, S Jeyakumar, N Ayyappan, N Adhirajan, G Premkumar, K Rajarathinam, S Muthuramkumar. 2016. Antimicrobial and cytotoxic activities of endophytic fungus Colletotrichum gloeosporioides isolated from endemic tree Cinnamomum malabatrum. Studies in Fungi 1(1):104−113 doi: 10.5943/sif/1/1/10
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