Figures (6)  Tables (1)

    • Figure 1. 

      Represents the 3D structures of ten MiAPX genes which were generated using SWISS-MODEL.

    • Figure 2. 

      Comparative analysis of APX gene motifs, domains, and structures. (a) Boxes of different colors represent the ten conserved motifs. (b) APX family protein domains. (c) The UTR–exon organization of APX genes, with UTRs shown in green, and exons (CDS) shown in yellow. Introns are represented by black lines connecting the exons.

    • Figure 3. 

      (a) The distribution of ten MiAPX genes across ten mango chromosomes is shown, with genes marked in red and chromosomes in black. (b) A circos plot illustrates the duplicated MiAPX gene pairs within the mango genome. (c) Syntemic relationships between APX genes in mango and A. thaliana were shown, with orange and green boxes representing the respective genomes. Red lines highlight conserved orthologous gene pairs.

    • Figure 4. 

      Phylogenetic tree of MiAPX genes from A. thaliana, rice, and mango. The tree was constructed using the maximum likelihood method with 1,000 bootstrap replications in MEGA 11. Different colors represent distinct groups.

    • Figure 5. 

      The Cis-acting elements in the mango promoter, with altered colours representing each element and their respective proportions.

    • Figure 6. 

      (a) Heatmap showing the expression patterns of MiAPX genes in response to UV-B stress. (b) qRT-PCR validation of ten MiAPX genes in samples collected at 30, 40, and 91 d after flowering under UV-B treatment. The x-axis represents days after flowering (d), and the y-axis indicates the relative expression levels of APX transcripts.

    • Gene IDs Given name A.A MW (kDa) pI Instability index Aliphatic index Gravy Subcellular localization
      LOC123198607.1 MiAPX1 246 27,152.47 5.38 39.61 81.34 −0.433 Peroxisome
      LOC123212144.1 MiAPX2 289 31,770.3 7.69 37.02 86.85 −0.272 Peroxisome
      LOC123214773.1 MiAPX3 286 31,561.02 6.67 36.65 84.93 −0.325 Peroxisome
      LOC123228152.1 MiAPX4 286 31,574.88 6.72 36.32 83.92 −0.366 Peroxisome
      LOC123201325.1 MiAPX5 250 27,500.05 5.38 32.41 80.04 −0.388 Peroxisome
      LOC123209556.1 MiAPX6 245 26,945.6 5.04 32.41 79.71 −0.255 Peroxisome
      LOC123224991.1 MiAPX7 249 27,394.17 5.52 28.7 78.84 −0.33 Peroxisome
      LOC123200457.1 MiAPX8 240 26,441.18 5.39 33.99 91.12 −0.135 Peroxisome
      LOC123203234.1 MiAPX9 621 69,336.45 5.9 48.18 75.3 −0.526 Chloroplast, mitochondrion

      Table 1. 

      Biological features of the APX gene family.