An iron sensor-based approach discovers a role for epigenetic regulation in iron homeostasis

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Figure 1.
Integrating genome-wide CRISPR screening and metabolic sensors to reveal cellular iron regulatory networks. (a) Genome-wide CRISPR screening with IRP2-Clover reporter cells revealed classical pathways and new regulators of cellular iron homeostasis. (b) A suggested paradigm that has the SETD2-H3K36me3 axis in the regulation of ferroptosis sensitivity, ferritinophagy, and IRP2. (c) Model for use of the fluorescent-fused metabolic sensor as a proxy for metabolite levels to screen and identify the upstream network and potential targetome. Note: figure was created using BioRender.com.