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    • Figure 1. 

      Ethylene content and the expression and subcellular location of StbZIP53-like2. (a) Ethylene content. (b) Expression profiles of 11 StbZIP genes in the RNA-Seq analysis during storage. (c) The expression of StbZIP53-like2. The expression levels of StbZIP53-like2 at different points are relative to 0 h, which was set as 1. *** indicates a significant difference at p < 0.001. (d) The subcellular location of StbZIP53-like2. Bars = 50 μm.

    • Figure 2. 

      Effect of StbZIP53-like2 mediates the transcriptional activity of StPPO2, StPPO3 and StPPO7. (a) Picture of the reporters and the effector. (b) The promoter activity of StPPO2, StPPO3 and StPPO7 was regulated by StbZIP53-like2. The empty vector co-expressed with promoters was used as a control (set as 1). *** indicates a significant difference at p < 0.001. (c) The cooperation of StbZIP53-like2 with the promoter of StPPO3 in the EMSAs. GST-StbZIP53-like2 protein was mixed with the probes of StPPO3 including the C-box motif and the mutant probes AAAAAA, which are shown in red letters. ++ indicates increasing amounts of the probe; – represents absence; + represents presence. (d) StbZIP53-like2 interacts with the promoter of StPPO3 in vivo. Yeast growth assays indicated the interaction of StbZIP53-like2 with the promoters of StPPO3.

    • Figure 3. 

      The cooperation between StbZIP53-like2 and StERF091 proteins. (a) The Y2H assay confirmed the cooperation of StbZIP53-like2 and StERF091. Yeast cells grew on SD/-Leu-Trp-Ade-His- with 125 μM Aureobasidin A and turned blue in the presence of 4 mg/mL X-α-Gal; this served as a positive interaction. (b) GST pull-down assay of the cooperation between StbZIP53-like2 and StERF091. His-StERF091 protein was incubated with GST-StbZIP53-like2 or GST. The anti-His antibody and anti-GST antibody were used for the immunoblotting assays. (c) The co-immunoprecipitation assay confirmed the interaction between StbZIP53-like2 and StERF091. Here, – represents the absence and + represents the presence of the fusion proteins. The anti-Flag and anti-GFP antibodies were used for immunoprecipitation.

    • Figure 4. 

      The expression and subcellular location of StERF091. (a) The expression of StERF091. The expression levels of StERF091 at different points are relative to 0 h, set as 1. ** and *** indicate significant differences at p < 0.01 and p < 0.001, respectively. (b) The subcellular location of StERF091. Bars = 50 μm.

    • Figure 5. 

      StERF091 mediates the transcriptional activity of StPPO2, StPPO3 and StPPO7. (a) Picture of the reporters and the effector. (b) The promoter activity of StPPO2, StPPO3 and StPPO7 was regulated by StERF091. The empty vector co-expressed with the promoters was used as a control (set as 1). ** and *** indicate significant differences at p < 0.01 and p < 0.001. (c) The cooperation of StERF091 with the promoter of StPPO2 and StPPO3 in EMSAs. GST-StERF091 protein was mixed with the probes of StPPO2 and StPPO3 including the GCC-box motif and the mutant probes AAAAAA (shown in red letters). ++ indicates increasing amounts of the probe, – represents absence and + represents presence. (d) StERF091 interacts with the promoter of StPPO2 and StPPO3 in vivo. Yeast growth assays indicated the interaction of StERF091 with the promoters of StPPO2 and StPPO3.

    • Figure 6. 

      Combinatory effects of StbZIP53-like2 and StERF091 in mediating the expression of StPPO2 and StPPO3. The empty vector co-expressed with the promoters was used as a control (set as 1). The letters a, b, c and d indicate significant differences at p < 0.05.

    • Figure 7. 

      Glu alleviates the browning of fresh-cut potatoes by enhancing the expression of StbZIP53-like2 and StERF091, thus inhibiting the transcriptional activity of StPPO2 and StPPO3.