Figures (9)  Tables (1)
    • Figure 1. 

      Three developmental stages of four varieties of pitaya. (For 'Jinduyihao', 'Yuenanbairou', and 'Wucihuanglong', the green fruit stage occurred at 20 DAP, the color transition stage at 25 DAP, and the mature stage at 30 DAP. In contrast, for 'Yanwoguo', the green fruit stage occurred at 90 DAP, the color transition stage at 105 DAP, and the mature stage at 120 DAP).

    • Figure 2. 

      (a) PCA map of the transcriptome; (b) PCA map of the metabolome.

    • Figure 3. 

      (a) Transcriptome KEGG enrichment analysis. (b) Metabolome KEGG enrichment analysis.

    • Figure 4. 

      DAMs and DEGs in the betalain metabolic pathway of pitaya peel. (a) Expression analysis of betalain synthesis pathway genes in four pitaya varieties at three periods of time. (b) Heat map of metabolite content clustering of betalain synthesis pathway.

    • Figure 5. 

      DAMs and DEGs in the flavonoid synthesis pathway of pitaya peel. (a) Expression analysis of flavonoid synthesis pathway genes in four pitaya varieties at three periods of time. (b) Clustered heat map of metabolite content of flavonoid synthesis pathway.

    • Figure 6. 

      DAMs and DEGs in the carotenoid synthesis pathway of pitaya peel. (a) Expression analysis of carotenoid synthesis pathway genes in four pitaya varieties at three time periods. (b) Clustered heat map of metabolite content of carotenoid synthesis pathway.

    • Figure 7. 

      DAMs and DEGs in the chlorophyll metabolism pathway of pitaya peel. (a) Expression analysis of chlorophyll metabolism pathway genes in four pitaya varieties at three periods of time. (b) Clustered heat map of metabolite content of chlorophyll metabolic pathways.

    • Figure 8. 

      Pitaya peel color-related DEGs qRT-PCR detection.

    • Figure 9. 

      Proposed regulatory model of pigment metabolic pathways underlying peel color differentiation in pitaya.

    • Genetic types Gene ID Forward primer (5'→3') Reverse primer (5'→3')
      CYP76DA1 NewGene_3232 GGCAACCACCTCACTCAACT CCAAGCAAGATCCTTAAACTCC
      B6GT HU06G00241 TGGTTGATGCGGACAGGG AAACGGCGACGGTATGAC
      cDOPA5GT HU05G00159 CTGCCTCATTGGTTTGCG ATGGGCTCATCTGGTTGC
      CHS HU04G00649 TTTCGCTATCCTTGTCGT CATCTCGTTGGGTGGGTT
      CHS HU04G00651 CTGAGGTGCCGAGATTGG TTGACAGATGGGCGAAGT
      CHI HU04G01107 CCACTACACCCGTTCGTT CTGGTCGCAGGTTCTTCT
      F3H HU06G02461 ACGGTACAAGAGGGCAGAC AGCGGGTAGACTTTAGCATC
      PSY HU03G00957 TTTGGATAGGTGGGAGGC ACGGCTGAATGTCAATAGGA
      HEMA HU02G01226 ATCTGTAGGTCCTGAAGTGG ATTGTAAACCCGAGCATT
      PAO HU02G03416 CCACAATTTCACTCCCTG ATCGGTTGGTCTGGTTAG
      Reference gene UBQ TGAATCATCCGACACCAT TCCTCTTCTTAGCACCACC

      Table 1. 

      Primer information of differentiated genes related to skin color of pitaya.