Figures (6)  Tables (2)

    • Figure 1. 

      Phylogenetic tree of MiLACs.

    • Figure 2. 

      Gene structure and motif analysis of MiLAC.

    • Figure 3. 

      Chromosome distribution and collinearity analysis of the MiLACs.

    • Figure 4. 

      Analysis of cis-acting elements of MiLACs promoter.

    • Figure 5. 

      q-PCR results of MiLACs under enhanced UV-B radiation treatment. Note: the two images in the first row are from 2023, and the two images in the second row are from 2024.

    • Figure 6. 

      Effect of enhanced UV-B radiation treatment on ABA and MeJA content changes in the pulp of 'Tainongyihao' mango.

    • Gene ID Gene name Number of
      amino acid      		 Molecular
      weight      		 pI Instability index Aliphatic index Grand average of
      hydropathicity      		 Signal
      peptide      		 Subcellular localization
      LOC123201611.1 MiLAC1 556 60,749.63 9.08 35.24 88.29 −0.041 YES chlo
      LOC123205456.1 MiLAC2 584 64,386.08 9.52 29.79 83.75 −0.076 YES chlo
      LOC123229717.1 MiLAC3 1147 126,257.25 5.19 34.44 80.51 −0.092 NO plas
      LOC123224994.1 MiLAC4 572 63,281.91 9.1 27.84 89.3 0.014 YES chlo
      LOC123209589.1 MiLAC5 578 64,417.75 8.48 36 78.58 −0.149 YES extr
      LOC123192083.1 MiLAC6 572 63,621.29 10.07 27.3 86.89 −0.091 YES chlo
      LOC123209528.1 MiLAC7 553 61,191.25 8.47 36.03 89.51 −0.056 YES vacu
      LOC123216306.1 MiLAC8 572 63,469.53 6.37 39.47 84.84 −0.043 YES chlo
      LOC123228608.1 MiLAC9 566 63,672.79 6.67 31.23 83.14 −0.127 YES plas
      LOC123229715.1 MiLAC10 574 64,038.58 6.59 33.01 76.74 −0.194 YES pero
      LOC123229713.1 MiLAC11 567 63,081.24 5.7 31.88 79.58 −0.146 YES vacu
      LOC123229041.1 MiLAC12 489 54,460.42 5.51 32.9 79.71 −0.18 NO cyto
      LOC123192537.1 MiLAC13 555 60,981.79 9.05 25.26 88.52 −0.045 YES extr
      LOC123198235.1 MiLAC14 568 63,437.73 6.37 34.2 77.2 −0.171 YES vacu
      LOC123229003.1 MiLAC15 561 62,323.26 6.07 33.51 82.51 −0.098 YES vacu
      LOC123201553.1 MiLAC16 557 60,936.06 9.09 33.74 90.09 −0.023 YES chlo
      LOC123228569.1 MiLAC17 582 65,830.37 5.57 38.25 78.83 −0.237 YES nucl
      LOC123206493.1 MiLAC18 568 63,338.6 6.37 35.12 77.2 −0.183 YES vacu
      LOC123229720.1 MiLAC19 568 63,373.65 6.54 33.88 76.51 −0.18 YES vacu
      LOC123203686.1 MiLAC20 561 62,591.07 6.43 35.67 76.45 −0.15 YES pero
      LOC123201619.1 MiLAC21 555 60,688.79 9.33 35.32 88.13 −0.055 YES chlo
      LOC123204379.1 MiLAC22 582 64,553.83 9.01 33.26 78.61 −0.161 YES chlo
      LOC123229716.1 MiLAC23 570 63,427.87 6.75 33.8 79.33 −0.175 YES chlo
      LOC123229257.1 MiLAC24 563 63,486.69 8.41 34.99 82.4 −0.168 YES chlo
      LOC123229178.1 MiLAC25 568 63,154.61 6.53 33.09 77.04 −0.177 YES chlo
      LOC123229004.1 MiLAC26 560 62,105.23 6.2 33.31 82.84 −0.088 YES vacu
      LOC123228609.1 MiLAC27 561 62,326.46 6.02 34.23 83.39 −0.077 YES vacu
      LOC123206108.1 MiLAC28 572 63,240.64 8.74 31.83 87.9 −0.034 YES chlo
      LOC123201844.1 MiLAC29 578 63,603.97 9.14 28.91 83.49 −0.063 YES chlo
      LOC123229714.1 MiLAC30 549 60,833.73 6.05 35.5 77.58 −0.215 NO cyto
      LOC123227135.1 MiLAC31 574 63,105.94 7.63 31.52 81.86 −0.051 YES chlo
      LOC123229712.1 MiLAC32 561 62,131.03 5.69 34.2 83.58 −0.074 YES vacu
      LOC123229362.1 MiLAC33 582 65,859.48 5.35 40.64 83.38 −0.204 YES nucl
      LOC123229040.1 MiLAC34 568 63,650.32 8.78 35.14 76.02 −0.275 NO vacu
      LOC123192675.1 MiLAC35 574 63,699.53 6.4 31.04 76.92 −0.073 YES chlo
      LOC123230046.1 MiLAC36 580 65,597.04 5.53 37.77 78.97 −0.261 YES extr
      LOC123198865.1 MiLAC37 561 62,316.52 7.69 35.58 84.3 −0.121 YES chlo
      LOC123201999.1 MiLAC38 566 62,256.27 8.69 30.64 85.92 −0.021 YES vacu
      LOC123229061.1 MiLAC39 564 62,158.85 4.83 35.2 78.67 −0.105 YES extr
      LOC123228568.1 MiLAC40 582 65,548.8 5.33 38.46 79.54 −0.267 YES nucl
      LOC123229436.1 MiLAC41 578 63,779.86 9.04 29.33 80.93 −0.097 YES chlo
      LOC123216462.1 MiLAC42 499 55,377.93 6.45 36.53 79.66 −0.117 NO cyto
      LOC123229209.1 MiLAC43 569 62,757.79 5.44 35.49 78.51 −0.093 NO extr
      LOC123206286.1 MiLAC44 552 61,357.32 7.66 36.69 86.5 −0.106 YES vacu
      LOC123229039.1 MiLAC45 569 63,648.25 6.1 35.15 79.02 −0.189 YES chlo
      LOC123229164.1 MiLAC46 569 62,698.66 5.52 35.51 79.02 −0.099 NO extr
      LOC123209471.1 MiLAC47 557 61,890.37 8.69 29.79 89.89 −0.036 YES chlo
      LOC123202218.1 MiLAC48 464 51,228.21 9.68 24.53 79.44 −0.176 NO cyto
      LOC123230053.1 MiLAC49 569 63,418 6.17 32.88 76.1 −0.202 YES E.R.
      LOC123229109.1 MiLAC50 569 63,430.15 6.62 32.22 76.1 −0.193 YES chlo
      LOC123216139.1 MiLAC51 566 62,425.18 8.43 32.58 83.22 −0.071 YES chlo
      LOC123208638.1 MiLAC52 589 65,038.69 8.96 32.73 87.57 −0.038 NO extr
      LOC123229718.1 MiLAC53 237 25,893.89 4.58 26.69 77.76 −0.093 NO cyto
      LOC123230055.1 MiLAC54 406 44,523.01 5.25 37.31 76.4 −0.138 NO cysk
      chlo: chloroplast; plas: plasma membrane; extr: extracellular; vacu: vacuole; cyto: cytoplasm; nucl: nucleus; pero: peroxisomes; E.R.: endoplasmic reticulum; cysk: cytoskeleton.

      Table 1. 

      Basic information on MiLAC proteins.

    • Seq_1 Seq_2 Ka Ks Ka/Ks
      MiLAC6 MiLAC2 0.205375182 1.971231992 0.104186206
      MiLAC2 MiLAC29 0.186898577 2.325797136 0.080358933
      MiLAC28 MiLAC4 0.050855744 0.360717481 0.140984973
      MiLAC6 MiLAC5 0.423715485 − −
      MiLAC5 MiLAC35 0.216972756 1.462202414 0.148387633
      MiLAC7 MiLAC13 0.265466731 1.557947571 0.170395163
      MiLAC14 MiLAC18 0.002294457 0.017957249 0.127773291
      MiLAC27 MiLAC20 0.133210788 0.511023074 0.260674703
      MiLAC31 MiLAC35 0.058176459 0.272809991 0.213249005
      MiLAC51 MiLAC38 0.080871157 0.579130917 0.139642272
      '−': the differences between sequences are too large, resulting in low similarity.

      Table 2. 

      Ka and Ks analysis of MiLACs replication gene pairs.