Figures (3)  Tables (2)

    • Figure 1. 

      M. anisopliae conidia production in different culture media. Columns with the same letter do not show significant differences (p < 0.05).

    • Figure 2. 

      (a) Mycelial growth of M. anisopliae from inside the insect T. molitor and (b) total invasion over its body.

    • Figure 3. 

      Mycosis caused by M. anisopliae in T. molitor. (a) Head, (b) cross-section, and (c) intersegmental folds of the cuticle of the insect.

    • Culture medium Composition (g/L)
      Commercial PDA Potato extract, 4; dextrose, 20
      MEA Maltose, 12.75; dextrin, 2.75; glycerol, 2.35; peptone, 0.78
      SDA Dextrose, 40; peptone, 10
      Laboratory-prepared formulations PDAy PDA with added yeast extract, 10
      A Dextrose, 40; yeast extract, 10; meat peptone, 10
      B Dextrose, 40; meat peptone, 10
      C Malt extract, 30; meat peptone, 5
      D Dextrose, 20; potato infusion, 200
      Media A, B, C, and D were supplemented with 15 g/L of agar.

      Table 1. 

      Culture media used for M. anisopliae conidia production.

    • Treatment t0 (days) k (days−1) LT50 (days)
      PDA 0.21 ± 0.02e 4.23 ± 0.14d 7.44 ± 0.27a
      MEA 0.35 ± 0.07d 5.43 ± 0.23b 7.69 ± 0.57a
      SDA 0.55 ± 0.04b 4.21 ± 0.03d 5.54 ± 0.42c
      PDAy 0.70 ± 0.09a 6.36 ± 0.23a 7.36 ± 0.20a
      A 0.54 ± 0.04b 3.96 ± 0.26d 5.27 ± 0.38c
      B 0.60 ± 0.01b 6.12 ± 0.33a 7.30 ± 0.36a
      C 0.48 ± 0.04c 4.80 ± 0.09c 6.53 ± 0.25b
      D 0.34 ± 0.07d 5.32 ± 0.32b 7.63 ± 0.26a
      The values of the columns with the same letter do not show significant differences (p < 0.05).

      Table 2. 

      Parameters of the bioassay with T. molitor infected with conidia of M. anisopliae obtained from different culture media.