[1]

Cocking EC. 1960. A method for the isolation of plant protoplasts and vacuoles. Nature 187:962−963

doi: 10.1038/187962a0
[2]

Carlson PS, Smith HH, Dearing RD. 1972. Parasexual interspecific plant hybridization. Proceedings of the National Academy of Sciences of the United States of America 69:2292−2294

doi: 10.1073/pnas.69.8.2292
[3]

Sheen J. 2001. Signal transduction in maize and Arabidopsis Mesophyll protoplasts. Plant Physiology 127:1466−1475

doi: 10.1104/pp.010820
[4]

Davey MR, Anthony P, Power JB, Lowe KC. 2005. Plant protoplasts: status and biotechnological perspectives. Biotechnology Advances 23:131−171

doi: 10.1016/j.biotechadv.2004.09.008
[5]

Xia G, Xiang F, Zhou A, Wang H, Chen H. 2003. Asymmetric somatic hybridization between wheat (Triticum aestivum L.) and Agropyron elongatum (Host) Nevishi. Theoretical and Applied Genetics 107:299−305

doi: 10.1007/s00122-003-1247-7
[6]

Lin YC, Li W, Chen H, Li Q, Sun YH, et al. 2014. A simple improved-throughput xylem protoplast system for studying wood formation. Nature Protocols 9:2194−2205

doi: 10.1038/nprot.2014.147
[7]

Sakhanokho HF, Islam-Faridi N, Babiker EM, Nelson CD, Stringer SJ, et al. 2020. Determination of nuclear DNA content, ploidy, and FISH location of ribosomal DNA in Hibiscus hamabo. Scientia Horticulturae 264:109167

doi: 10.1016/j.scienta.2019.109167
[8]

Wang Z, Xue JY, Hu SY, Zhang F, Yu R, et al. 2022. The genome of Hibiscus hamabo reveals its adaptation to saline and waterlogged habitat. Horticulture Research 9:uhac067

doi: 10.1093/hr/uhac067
[9]

Xu Y, Ni L, Yu C, Hua J, Yin Y, et al. 2025. Genome-wide study of the R2R3-MYB gene family and analysis of HhMYB111r-induced salt tolerance in Hibiscus hamabo Sieb. et Zucc. Plant Science 352:112378

doi: 10.1016/j.plantsci.2024.112378
[10]

Liu D, Gu C, Fu Z, Wang Z. 2023. Genome-wide identification and analysis of MYB transcription factor family in Hibiscus hamabo. Plants 12:1429

doi: 10.3390/plants12071429
[11]

Ni L, Wang Z, Liu X, Wu S, Hua J, et al. 2022. Genome-wide study of the GRAS gene family in Hibiscus hamabo Sieb. et Zucc and analysis of HhGRAS14-induced drought and salt stress tolerance in Arabidopsis. Plant Science 319:111260

doi: 10.1016/j.plantsci.2022.111260
[12]

Wang Z, Ni L, Liu D, Fu Z, Hua J, et al. 2022. Genome-wide identification and characterization of NAC family in Hibiscus hamabo Sieb. et Zucc. under various abiotic stresses. International Journal of Molecular Sciences 23:3055

doi: 10.3390/ijms23063055
[13]

Chen HC, Li Q, Shuford CM, Liu J, Muddiman DC, et al. 2011. Membrane protein complexes catalyze both 4- and 3-hydroxylation of cinnamic acid derivatives in monolignol biosynthesis. Proceedings of the National Academy of Sciences of the United States of America 108:21253−21258

doi: 10.1073/pnas.1116416109
[14]

Li Q, Lin YC, Sun YH, Song J, Chen H, et al. 2012. Splice variant of the SND1 transcription factor is a dominant negative of SND1 members and their regulation in Populus trichocarpa. Proceedings of the National Academy of Sciences of the United States of America 109:14699−14704

doi: 10.1073/pnas.1212977109
[15]

Lin YC, Li W, Sun YH, Kumari S, Wei H, et al. 2013. SND1 transcription factor–directed quantitative functional hierarchical genetic regulatory network in wood formation in Populus trichocarpa. The Plant Cell 25:4324−4341

doi: 10.1105/tpc.113.117697
[16]

Zhao YX, Yao DY, Harris PJC. 1991. Isolation and culture of protoplasts from callus tissue of Hibiscus syriacus L. Plant Cell, Tissue and Organ Culture 25:17−19

doi: 10.1007/BF00033907
[17]

Yoo SD, Cho YH, Sheen J. 2007. Arabidopsis mesophyll protoplasts: a versatile cell system for transient gene expression analysis. Nature Protocols 2:1565−1572

doi: 10.1038/nprot.2007.199
[18]

Ren R, Gao J, Yin D, Li K, Lu C, et al. 2021. Highly efficient leaf base protoplast isolation and transient expression systems for orchids and other important monocot crops. Frontiers in Plant Science 12:626015

doi: 10.3389/fpls.2021.626015
[19]

Cho YH, Yoo SD, Sheen J. 2006. Regulatory functions of nuclear hexokinase1 complex in glucose signaling. Cell 127:579−589

doi: 10.1016/j.cell.2006.09.028
[20]

Liu X, Liu H, Liu WC, Gao Z. 2020. The nuclear localized RIN13 induces cell death through interacting with ARF1. Biochemical and Biophysical Research Communications 527:124−130

doi: 10.1016/j.bbrc.2020.04.082
[21]

Yu J, Zhou C, Li D, Li S, Lin YCJ, et al. 2022. A PtrLBD39-mediated transcriptional network regulates tension wood formation in Populus trichocarpa. Plant Communications 3:100250

doi: 10.1016/j.xplc.2021.100250
[22]

Liu H, Gao J, Sun J, Li S, Zhang B, et al. 2022. Dimerization of PtrMYB074 and PtrWRKY19 mediates transcriptional activation of PtrbHLH186 for secondary xylem development in Populus trichocarpa. New Phytologist 234:918−933

doi: 10.1111/nph.18028
[23]

Chen H, Wang JP, Liu H, Li H, Lin YCJ, et al. 2019. Hierarchical transcription factor and chromatin binding network for wood formation in Populus trichocarpa. The Plant Cell 31:602−626

doi: 10.1105/tpc.18.00620
[24]

Ehlert A, Weltmeier F, Wang X, Mayer CS, Smeekens S, et al. 2006. Two-hybrid protein–protein interaction analysis in Arabidopsis protoplasts: establishment of a heterodimerization map of group C and group S bZIP transcription factors. The Plant Journal 46:890−900

doi: 10.1111/j.1365-313X.2006.02731.x
[25]

Chiu WL, Niwa Y, Zeng W, Hirano T, Kobayashi H, et al. 1996. Engineered GFP as a vital reporter in plants. Current Biology 6:325−330

doi: 10.1016/s0960-9822(02)00483-9
[26]

Yanagisawa S, Yoo SD, Sheen J. 2003. Differential regulation of EIN3 stability by glucose and ethylene signalling in plants. Nature 425:521−525

doi: 10.1038/nature01984
[27]

Kovtun Y, Chiu WL, Tena G, Sheen J. 2000. Functional analysis of oxidative stress-activated mitogen-activated protein kinase cascade in plants. Proceedings of the National Academy of Sciences of the United States of America 97:2940−2945

doi: 10.1073/pnas.97.6.2940
[28]

He P, Shan L, Lin NC, Martin GB, Kemmerling B, et al. 2006. Specific bacterial suppressors of MAMP signaling upstream of MAPKKK in Arabidopsis innate immunity. Cell 125:563−575

doi: 10.1016/j.cell.2006.02.047
[29]

Wang Z, Mao Y, Guo Y, Gao J, Liu X, et al. 2020. MYB transcription factor161 mediates feedback regulation of secondary wall-associated NAC-domain1 family genes for wood formation. Plant Physiology 184:1389−1406

doi: 10.1104/pp.20.01033
[30]

Ma H, Su L, Zhang W, Sun Y, Li D, et al. 2025. Epigenetic regulation of lignin biosynthesis in wood formation. New Phytologist 245:1589−1607

doi: 10.1111/nph.20328
[31]

Li S, Lin YCJ, Wang P, Zhang B, Li M, et al. 2019. The AREB1 transcription factor influences histone acetylation to regulate drought responses and tolerance in Populus trichocarpa. The Plant Cell 31:663−686

doi: 10.1105/tpc.18.00437
[32]

Zhang B, Wang Z, Dai X, Gao J, Zhao J, et al. 2024. A COMPASS histone H3K4 trimethyltransferase pentamer transactivates drought tolerance and growth/biomass production in Populus trichocarpa. New Phytologist 241:1950−1972

doi: 10.1111/nph.19481
[33]

Gao J, Wu X, Zhai R, Liu H, Zhao J, et al. 2025. Coordinated chromatin modifications mediated by AREB and MYB transcription factors sustain drought tolerance in Populus. Plant Physiology 198:kiaf271

doi: 10.1093/plphys/kiaf271
[34]

Dai X, Zhai R, Lin J, Wang Z, Meng D, et al. 2023. Cell-type-specific PtrWOX4a and PtrVCS2 form a regulatory nexus with a histone modification system for stem cambium development in Populus trichocarpa. Nature Plants 9:96−111

doi: 10.1038/s41477-022-01315-7
[35]

Geng L, Tan M, Deng Q, Wang Y, Zhang T, et al. 2024. Transcription factors WOX11 and LBD16 function with histone demethylase JMJ706 to control crown root development in rice. The Plant Cell 36:1777−1790

doi: 10.1093/plcell/koad318
[36]

Birnbaum K, Shasha DE, Wang JY, Jung JW, Lambert GM, et al. 2003. A gene expression map of the Arabidopsis root. Science 302:1956−1960

doi: 10.1126/science.1090022
[37]

Li H, Dai X, Huang X, Xu M, Wang Q, et al. 2021. Single-cell RNA sequencing reveals a high-resolution cell atlas of xylem in Populus. Journal of Integrative Plant Biology 63:1906−1921

doi: 10.1111/jipb.13159