[1]

Gamba M, Asllanaj E, Raguindin PF, Glisic M, Franco OH, et al. 2021. Nutritional and phytochemical characterization of radish (Raphanus sativus): a systematic review. Trends in Food Science & Technology 113:205−218

doi: 10.1016/j.tifs.2021.04.045
[2]

Singh BK. 2021. Radish (Raphanus sativus L.): breeding for higher yield, better quality and wider adaptability. In Advances in Plant Breeding Strategies: Vegetable Crops, eds. Al-Khayri JM, Jain SM, Johnson DV. Cham: Springer International Publishing. pp. 275−304 doi: 10.1007/978-3-030-66965-2_7
[3]

Ferrie AMR, Caswell KL. 2011. Isolated microspore culture techniques and recent progress for haploid and doubled haploid plant production. Plant Cell, Tissue and Organ Culture (PCTOC) 104:301−309

doi: 10.1007/s11240-010-9800-y
[4]

Shi C, Zhao Z, Zhong Y, Qiao Y, Zhang L, et al. 2025. Reprogramming of microspore fate via BBM-BAR1 for highly efficient in vivo haploid induction. Cell 188:6109−6120.e15

doi: 10.1016/j.cell.2025.08.014
[5]

Kelliher T, Starr D, Richbourg L, Chintamanani S, Delzer B, et al. 2017. MATRILINEAL, a sperm-specific phospholipase, triggers maize haploid induction. Nature 542:105−109

doi: 10.1038/nature20827
[6]

Li Y, Lin Z, Yue Y, Zhao H, Fei X, et al. 2021. Loss-of-function alleles of ZmPLD3 cause haploid induction in maize. Nature Plants 7:1579−1588

doi: 10.1038/s41477-021-01037-2
[7]

Gilles LM, Khaled A, Laffaire JB, Chaignon S, Gendrot G, et al. 2017. Loss of pollen‐specific phospholipase NOT LIKE DAD triggers gynogenesis in maize. The EMBO Journal 36:707−717

doi: 10.15252/embj.201796603
[8]

Kelliher T, Starr D, Wang W, McCuiston J, Zhong H, et al. 2016. Maternal haploids are preferentially induced by CENH3-tailswap transgenic complementation in maize. Frontiers in Plant Science 7:414

doi: 10.3389/fpls.2016.00414
[9]

Wang N, Gent JI, Dawe RK. 2021. Haploid induction by a maize cenh3 null mutant. Science Advances 7:eabe2299

doi: 10.1126/sciadv.abe2299
[10]

Zhong Y, Chen B, Li M, Wang D, Jiao Y, et al. 2020. A DMP-triggered in vivo maternal haploid induction system in the dicotyledonous Arabidopsis. Nature Plants 6:466−472

doi: 10.1038/s41477-020-0658-7
[11]

Cyprys P, Lindemeier M, Sprunck S. 2019. Gamete fusion is facilitated by two sperm cell-expressed DUF679 membrane proteins. Nature Plants 5:253−257

doi: 10.1038/s41477-019-0382-3
[12]

Kasaras A, Kunze R. 2010. Expression, localisation and phylogeny of a novel family of plant-specific membrane proteins. Plant Biology 12:140−152

doi: 10.1111/j.1438-8677.2010.00381.x
[13]

Kasaras A, Kunze R. 2017. Dual-targeting of Arabidopsis DMP1 isoforms to the tonoplast and the plasma membrane. PLoS One 12:e0174062

doi: 10.1371/journal.pone.0174062
[14]

van der Graaff E, Schwacke R, Schneider A, Desimone M, Flügge UI, et al. 2006. Transcription analysis of Arabidopsis membrane transporters and hormone pathways during developmental and induced leaf senescence. Plant Physiology 141:776−792

doi: 10.1104/pp.106.079293
[15]

Nawade B, Bosamia TC, Lee JH, Jang JH, Lee OR. 2023. Genome-wide characterization of the soybean DOMAIN OF UNKNOWN FUNCTION 679 membrane protein gene family highlights their potential involvement in growth and stress response. Frontiers in Plant Science 14:1216082

doi: 10.3389/fpls.2023.1216082
[16]

Sprunck S. 2020. Twice the fun, double the trouble: gamete interactions in flowering plants. Current Opinion in Plant Biology 53:106−116

doi: 10.1016/j.pbi.2019.11.003
[17]

Zhong Y, Liu C, Qi X, Jiao Y, Wang D, et al. 2019. Mutation of ZmDMP enhances haploid induction in maize. Nature Plants 5:575−580

doi: 10.1038/s41477-019-0443-7
[18]

Pan Z, Li Z, Han Y, Sun J. 2024. Genome-wide identification and expression analysis of the DMP and MTL genes in sweetpotato (Ipomoea batatas L.). Genes 15:354

doi: 10.3390/genes15030354
[19]

Tian S, Zhang J, Zhao H, Zong M, Li M, et al. 2023. Production of double haploid watermelon via maternal haploid induction. Plant Biotechnology Journal 21:1308−1310

doi: 10.1111/pbi.14045
[20]

Yin S, Li S, Sun L, Shi K, Fan S, et al. 2024. Mutating the maternal haploid inducer gene CsDMP in cucumber produces haploids in planta. Plant Physiology 194:1282−1285

doi: 10.1093/plphys/kiad600
[21]

Zhang J, Yin J, Luo J, Tang D, Zhu X, et al. 2022. Construction of homozygous diploid potato through maternal haploid induction. aBIOTECH 3:163−168

doi: 10.1007/s42994-022-00080-7
[22]

Li Y, Li D, Xiao Q, Wang H, Wen J, et al. 2022. An in planta haploid induction system in Brassica napus. Journal of Integrative Plant Biology 64:1140−1144

doi: 10.1111/jipb.13270
[23]

Chen L, Wang S, Zhang YH, Li J, Xing ZH, et al. 2017. Identify key sequence features to improve CRISPR sgRNA efficacy. IEEE Access 5:26582−26590

doi: 10.1109/ACCESS.2017.2775703
[24]

Preece R, Georgiadis C, Gkazi SA, Etuk A, Christi A, et al. 2020. 'Mini'U6 Pol III promoter exhibits nucleosome redundancy and supports multiplexed coupling of CRISPR/Cas9 effects. Gene Therapy 27:451−458

doi: 10.1038/s41434-020-0142-z
[25]

Long L, Guo DD, Gao W, Yang WW, Hou LP, et al. 2018. Optimization of CRISPR/Cas9 genome editing in cotton by improved sgRNA expression. Plant Methods 14:85

doi: 10.1186/s13007-018-0353-0
[26]

Charrier A, Vergne E, Dousset N, Richer A, Petiteau A, et al. 2019. Efficient targeted mutagenesis in apple and first time edition of pear using the CRISPR-Cas9 system. Frontiers in Plant Science 10:40

doi: 10.3389/fpls.2019.00040
[27]

Zhou J, Wang G, Liu Z. 2018. Efficient genome editing of wild strawberry genes, vector development and validation. Plant Biotechnology Journal 16:1868−1877

doi: 10.1111/pbi.12922
[28]

Yang W, Ren J, Liu W, Liu D, Xie K, et al. 2023. An efficient transient gene expression system for protein subcellular localization assay and genome editing in citrus protoplasts. Horticultural Plant Journal 9:425−436

doi: 10.1016/j.hpj.2022.06.006
[29]

Wang Y, Zhang YA, Dong Y, Li D, Shi S, et al. 2022. A highly efficient mesophyll protoplast isolation and PEG-mediated transient expression system in eggplant. Scientia Horticulturae 304:111303

doi: 10.1016/j.scienta.2022.111303
[30]

Reiser L, Bakker E, Subramaniam S, Chen X, Sawant S, et al. 2024. The Arabidopsis information resource in 2024. Genetics 227:iyae027

doi: 10.1093/genetics/iyae027
[31]

Xu L, Wang Y, Dong J, Zhang W, Tang M, et al. 2023. A chromosome-level genome assembly of radish (Raphanus sativus L.) reveals insights into genome adaptation and differential bolting regulation. Plant Biotechnology Journal 21:990−1004

doi: 10.1111/pbi.14011
[32]

Bateman A, Coin L, Durbin R, Finn RD, Hollich V, et al. 2004. The Pfam protein families database. Nucleic Acids Research 32:D138−D141

doi: 10.1093/nar/gkh121
[33]

Gasteiger E, Hoogland C, Gattiker A, Duvaud S, Wilkins MR, et al. 2005. Protein identification and analysis tools on the ExPASy server. In The Proteomics Protocols Handbook, ed. Walker JM. Totowa, NJ: Humana Press. pp. 571−607 doi: 10.1385/1-59259-890-0:571
[34]

Thumuluri V, Almagro Armenteros JJ, Johansen AR, Nielsen H, Winther O. 2022. DeepLoc 2.0: multi-label subcellular localization prediction using protein language models. Nucleic Acids Research 50:W228−W234

doi: 10.1093/nar/gkac278
[35]

Hallgren J, Tsirigos KD, Pedersen MD, Almagro Armenteros JJ, Marcatili P, et al. 2022. DeepTMHMM predicts alpha and beta transmembrane proteins using deep neural networks. bioRxiv 1−12

doi: 10.1101/2022.04.08.487609
[36]

Chen C, Chen H, Zhang Y, Thomas HR, Frank MH, et al. 2020. TBtools: an integrative toolkit developed for interactive analyses of big biological data. Molecular Plant 13:1194−1202

doi: 10.1016/j.molp.2020.06.009
[37]

Bailey TL, Williams N, Misleh C, Li WW. 2006. MEME: discovering and analyzing DNA and protein sequence motifs. Nucleic Acids Research 34:W369−W373

doi: 10.1093/nar/gkl198
[38]

Hu B, Jin J, Guo AY, Zhang H, Luo J, et al. 2015. GSDS 2.0: an upgraded gene feature visualization server. Bioinformatics 31:1296−1297

doi: 10.1093/bioinformatics/btu817
[39]

Xie J, Chen Y, Cai G, Cai R, Hu Z, et al. 2023. Tree Visualization By One Table (tvBOT): a web application for visualizing, modifying and annotating phylogenetic trees. Nucleic Acids Research 51:W587−W592

doi: 10.1093/nar/gkad359
[40]

Tamura K, Stecher G, Kumar S. 2021. MEGA11: molecular evolutionary genetics analysis version 11. Molecular Biology and Evolution 38:3022−3027

doi: 10.1093/molbev/msab120
[41]

Livak KJ, Schmittgen TD. 2001. Analysis of relative gene expression data using real-time quantitative PCR and the 2−ΔΔCᴛ method. Methods 25:402−408

doi: 10.1006/meth.2001.1262
[42]

Yoo SD, Cho YH, Sheen J. 2007. Arabidopsis mesophyll protoplasts: a versatile cell system for transient gene expression analysis. Nature Protocols 2:1565−1572

doi: 10.1038/nprot.2007.199
[43]

Yu X, Yu J, Lu Y, Li W, Huo G, et al. 2024. An efficient and universal protoplast-based transient gene expression system for genome editing in Brassica crops. Horticultural Plant Journal 10:983−994

doi: 10.1016/j.hpj.2024.06.001
[44]

Porebski S, Bailey LG, Baum BR. 1997. Modification of a CTAB DNA extraction protocol for plants containing high polysaccharide and polyphenol components. Plant Molecular Biology Reporter 15:8−15

doi: 10.1007/BF02772108
[45]

Clement K, Rees H, Canver MC, Gehrke JM, Farouni R, et al. 2019. Accurate and rapid analysis of genome editing data from nucleases and base editors with CRISPResso2. Nature Biotechnology 37:224−226

doi: 10.1038/s41587-019-0032-3
[46]

Li L, Hou S, Xiang W, Song Z, Wang Y, et al. 2022. The egg cell is preferentially fertilized in Arabidopsis double fertilization. Journal of Integrative Plant Biology 64:2039−2046

doi: 10.1111/jipb.13370
[47]

Zhong Y, Chen B, Wang D, Zhu X, Li M, et al. 2022. In vivo maternal haploid induction in tomato. Plant Biotechnology Journal 20:250−252

doi: 10.1111/pbi.13755
[48]

Liu C, Li X, Meng D, Zhong Y, Chen C et al. 2017. A 4-bp insertion at ZmPLA1 encoding a putative phospholipase a generates haploid induction in maize. Molecular Plant 10:520−522

doi: 10.1016/j.molp.2017.01.011
[49]

Shao J, Peng B, Zhang Y, Yan X, Yao X, et al. 2024. A high-efficient protoplast transient system for screening gene editing elements in Salvia miltiorrhiza. Plant Cell Reports 43:45

doi: 10.1007/s00299-023-03134-2
[50]

Li F, Xue M, Guo D, Zhu L, Li Y, et al. 2025. A truncated endogenous U6 promoter enables high-efficiency CRISPR editing in flax (Linum usitatissimum L.). Plants 14:1142

doi: 10.3390/plants14071142
[51]

Bian S, Han X, Yuan G, Zhang L, Tian Y, et al. 2019. Cloning and functional analysis of U6 promoter in apple. Scientia Agricultura Sinica. 52:4364−4373 (in Chinese)

doi: 10.3864/j.issn.0578-1752.2019.23.016