Chemical compositions, cytotoxicity and antioxidant activity of the endophytic fungus Fusarium napiforme isolated from Psidium guajava
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1.
Department of Studies & Research in Microbiology, Mangalore University, Jnana Kaveri, P. G. Centre, Chikka Aluvara, Kodagu-571232, Karnataka, India
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2.
Department of Biosciences, Mangalore University, Mangalagangotri-574199, Karnataka, India
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3.
Department of Biology, Wolaita Sodo University, Wolaita Sodo, Ethiopia
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Department of Applied Botany, Mangalore University, Mangalagangotri-574199, Karnataka, India
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5.
Department of Biochemistry, CSIR-Central Food Technological Research Institute, Mysuru-570020, Karnataka, India
More Information
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Received:
27 January 2020
Revised:
08 June 2020
Published online:
17 June 2020
Studies in Fungi
5(1): 332−352(2020) |
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Abstract
The bioactive secondary metabolites from the endophytic fungus Fusarium napiforme was evaluated for the cytotoxic effect and antioxidant activity. The total antioxidant capacity (TAC) of the extract was determined by 2, 2-diphenyl-1-picrylhydrazyl (DPPH), phosphomolybdate, and reducing power assay methods. The cytotoxicity of the extract was evaluated against lung adenocarcinoma (A549) cells and mouse embryo fibroblast (NIH3T3) cells by methyl thiazolyl tetrazolium (MTT) method. The major composition of the crude extract was identified by the Gas Chromatography-Mass Spectrometry (GC-MS) analysis. Estimation of the endophyte crude extract revealed a high amount of the total flavonoid content (TFC) and total phenolic content (TPC). The extract showed high cytotoxic activity against the A549 cell line with the mean cytotoxicity of 69.74 ±0.49%. The extract did not show any cytotoxic effect against the NIH3T3 cell line. The extract exhibited high antioxidant activity as a function of the concentrations. At a test concentration of 1 mg ml-1, the extract showed the highest inhibition against DPPH radical at 75.4607%±0.47688, ferric ion reducing power at 0.882±0.0120, and 255.434±21.404 AAE/g of the extract by phosphomolybdenum assay (PMA). There is a significant correlation between TPC and antioxidant activity at p < 0.05. The correlation between reducing power and DPPH is significant at p < 0.01. The major types of bioactive compounds identified by the GC-MS have shown the presence of nine major compounds. This result strongly exhibits that the endophyte F. napiforme can be a potential source for the formulation of natural anticancer drugs and protecting the body from oxidative damages.
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Copyright: © 2020 by the author(s). This article is an open access article distributed under Creative Commons Attribution License (CC BY 4.0), visit https://creativecommons.org/licenses/by/4.0/.
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EC Chutulo, RK Chalannavar, P Pramod Kumar. 2020. Chemical compositions, cytotoxicity and antioxidant activity of the endophytic fungus Fusarium napiforme isolated from Psidium guajava. Studies in Fungi 5(1):332−352 doi: 10.5943/sif/5/1/15
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EC Chutulo, RK Chalannavar, P Pramod Kumar. 2020. Chemical compositions, cytotoxicity and antioxidant activity of the endophytic fungus Fusarium napiforme isolated from Psidium guajava. Studies in Fungi 5(1):332−352 doi: 10.5943/sif/5/1/15
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